| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHgA7 |
| Uniprot No | Q9Y5G6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-101aa |
| 氨基酸序列 | MAAQPRGGDYRGFFLLSILLGTPWEAWAGRILYSVSEETDKGSFVGDIAKDLGLEPRELAERGVRIISRGRTQLFALNQRSGSLVTAGRIDREEICAQSARCLVNFNILMEDKMNLYPIDVEIIDINDNVPRFLTEEINVKIMENTAPGVRFPLSEAGDPDVGTNSLQSYQLSPNRHFSLAVQSGDDETKYPELVLERVLDREEERVHHLVLTASDGGDPPRSSTAHIQVTVVDVNDHTPVFSLPQYQVTVPENVPVGTRLLTVHAIDLDEGVNGEVTYSFRKITPKLPKMFHLNSLTGEISTLEGLDYEETAFYEMEVQAQDGPGSLTKAKVLITVLDVNDNAPEVTMTSLSSSIPEDTPLGTVIALFYLQDRDSGKNGEVTCTIPENLPFKLEKSIDNYYRLVTTKNLDRETLSLYNITLKATDGGTPPLSRETHIFMQVADTNDNPPTFPHSSYSVYIAENNPRGASIFLVTAQDHDSEDNAQITYSLAEDTIQGAPVSSYVSINSDTGVLYALQSFDYEQLRELQLRVTAHDSGDPPLSSNMSLSLFVLDQNDNPPEILYPALPTDGSTGMELAPRSAEPGYLVTKVVAVDKDSGQNAWLSYLLLKASEPGLFAVGLYTGEVRTARALLDRDALKQSLVVAVQDHGQPPLSATVTLTVAVADSIPEVLADLGSLEPSDGPYNYDLTLYLVVAVATVSCVFLAFVLVLLALRLRRWHKSRLLQASEGGLANVPTSHFVGMDGVQAFLQTYSHEVSLTADSRKSHLIFPQPNYVDMLISQESCEKNDSLLTSVDFQECKENLPSIQQAPPNTDWRFSQAQRPGTSGSQNGDDTGTWPNNQFDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQFTLQHVPDYRQNVYIPGSNATLTNAAGKRDGKAPAGGNGNKKKSGKKEKK |
| 预测分子量 | 101 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCDHGA7重组蛋白的模拟参考文献示例(注：内容为假设性概括，非真实文献)：
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1. **文献名称**: *"Recombinant PCDHGA7 Protein Expression and Its Role in Neuronal Adhesion"*
**作者**: Smith J, et al.
**摘要**: 研究利用大肠杆菌系统表达重组PCDHGA7蛋白，验证其在神经元细胞粘附中的作用，证实其通过钙离子依赖性机制促进突触形成。
2. **文献名称**: *"Structural Analysis of PCDHGA7 Extracellular Domain Using Cryo-EM"*
**作者**: Johnson L, et al.
**摘要**: 通过冷冻电镜解析重组PCDHGA7胞外结构域的三维构象，揭示其特异性同源结合特性，为神经发育疾病机制提供结构基础。
3. **文献名称**: *"PCDHGA7 Recombinant Protein as a Biomarker in Glioblastoma"*
**作者**: Lee S, et al.
**摘要**: 开发基于重组PCDHGA7的ELISA检测方法，发现其在胶质母细胞瘤患者血清中异常高表达，提示其作为潜在诊断标志物。
4. **文献名称**: *"CRISPR/Cas9-Mediated Knockout and Recombinant Rescue of PCDHGA7 in Autism Models"*
**作者**: Zhang Y, et al.
**摘要**: 利用重组PCDHGA7蛋白挽救自闭症模型小鼠的神经元连接缺陷，证明其在神经环路发育中的关键调控作用。
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(注：以上文献信息为虚构，仅供示例参考。实际研究中请通过学术数据库检索真实文献。)
**Background of PCDHgA7 Recombinant Protein**
The protocadherin gamma subfamily A7 (PCDHgA7) is a member of the clustered protocadherin (PCDH) family, a group of transmembrane proteins critical for neuronal development, synaptic specificity, and cell-cell adhesion in the central nervous system. As part of the cadherin superfamily, PCDHgA7 contains extracellular cadherin repeats that mediate calcium-dependent homophilic interactions, a transmembrane domain, and a conserved cytoplasmic tail. Its expression is regulated by a combination of promoter choice and alternative splicing, contributing to the remarkable diversity required for neural circuit assembly and synaptic plasticity.
PCDHgA7 recombinant protein is engineered to study the structure-function relationships and biological roles of this isoform in vitro or in vivo. Produced via recombinant DNA technology in systems like *E. coli* or mammalian cell cultures, the protein often includes epitope tags (e.g., His-tag, FLAG) for purification and detection. Researchers utilize this tool to investigate PCDHgA7’s involvement in neurodevelopmental processes, such as axon guidance, dendritic arborization, and synapse formation. Dysregulation of PCDHgA7 has been implicated in neurological disorders, including autism spectrum disorders and schizophrenia, as well as cancer progression due to altered cell adhesion and signaling pathways.
Additionally, recombinant PCDHgA7 enables biochemical assays, binding partner identification, and antibody development. Its role in homophilic or heterophilic interactions with other PCDH isoforms or cell surface receptors (e.g., Wnt or tyrosine kinase pathways) remains a key focus, offering insights into disease mechanisms and therapeutic targeting. Studies using animal models or neuronal cultures often rely on recombinant proteins to dissect isoform-specific functions, highlighting PCDHgA7’s unique contributions to neural connectivity and tissue homeostasis.
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