| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPSA |
| Uniprot No | P08865 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-295aa |
| 氨基酸序列 | SGALDVLQMKEEDVLKFLAAGTHLGGTNLDFQMEQYIYKRKSDGIYIINLKRTWEKLLLAARAIVAIENPADVSVISSRNTGQRAVLKFAAATGATPIAGRFTPGTFTNQIQAAFREPRLLVVTDPRADHQPLTEASYVNLPTIALCNTDSPLRYVDIAIPCNNKGAHSVGLMWWMLAREVLRMRGTISREHPWEVMPDLYFYRDPEEIEKEEQAAAEKAVTKEEFQGEWTAPAPEFTATQPEVADWSEGVQVPSVPIQQFPTEDWSAQPATEDWSAAPTAQATEWVGATTDWS |
| 预测分子量 | 76.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于RPSA重组蛋白的参考文献及摘要概括：
---
1. **文献名称**：*"Recombinant Ribosomal Protein SA as a Potential Receptor for Dengue Virus"*
**作者**：Tio, P. H., et al.
**摘要**：研究通过重组表达RPSA蛋白，验证其作为登革热病毒受体的功能。实验表明，重组RPSA能与病毒包膜蛋白直接结合，并通过抗体阻断实验证实其在病毒入侵宿主细胞中的关键作用。
---
2. **文献名称**：*"Structural Insights into the Laminin Receptor Ribosomal Protein SA by Recombinant Expression and Crystallography"*
**作者**：Ardini, E., et al.
**摘要**：利用大肠杆菌系统重组表达并纯化RPSA蛋白，通过X射线晶体学解析其三维结构，揭示了其与层粘连蛋白结合的特定结构域，为研究其在肿瘤转移中的分子机制提供依据。
---
3. **文献名称**：*"Functional Analysis of Recombinant 37LRP in HCV Entry"*
**作者**：Mazier, D., et al.
**摘要**：通过哺乳动物细胞表达重组RPSA(37LRP)，发现其与丙肝病毒(HCV)颗粒特异性结合，并通过siRNA敲低实验证明RPSA是HCV感染肝细胞的重要辅助受体。
---
**备注**：上述文献为示例性质，实际引用时建议通过PubMed或Google Scholar核对具体信息。
**Background of RPSA Recombinant Protein**
The Ribosomal Protein SA (RPSA), also known as LamR (Laminin Receptor 1), is a multifunctional protein encoded by the *RPSA* gene. Initially identified as a component of the 40S ribosomal subunit, RPSA plays a critical role in ribosome assembly and protein biosynthesis. Beyond its ribosomal function, RPSA is a cell-surface receptor for laminin, a key extracellular matrix protein involved in cell adhesion, migration, and differentiation. This dual role—intracellular in ribosome function and extracellular in laminin signaling—underscores its biological versatility.
RPSA has garnered attention in biomedical research due to its involvement in various pathological processes. It is overexpressed in multiple cancers, where it promotes tumor progression, metastasis, and angiogenesis by interacting with laminin and other ligands. Additionally, RPSA serves as a receptor for pathogens, including *Dengue virus* and prion proteins, facilitating their cellular entry. Its implication in neurodegenerative diseases, such as Alzheimer’s, further highlights its therapeutic relevance.
Recombinant RPSA protein is engineered using expression systems like *E. coli* or mammalian cells, enabling large-scale production for functional studies. Purified through affinity chromatography, it retains structural and functional integrity, making it valuable for investigating molecular mechanisms, protein interactions, and signaling pathways. Researchers utilize RPSA recombinant protein to develop targeted therapies, diagnostic tools, and vaccines, leveraging its role in disease pathways. Its application in drug delivery systems, exploiting laminin-binding properties, also holds promise for precision medicine.
Overall, RPSA recombinant protein serves as a pivotal tool in unraveling the protein’s multifunctional biology and advancing translational research across oncology, virology, and neurology.
×
