| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Sprr2b |
| Uniprot No | P35325 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-72aa |
| 氨基酸序列 | MSYQQQQCKQPCQPPPVCPTPKCPEPCPPPKCPEPCPPPKCPQPCPPQQCQQKYPPVTPSPPCQPKYPPKSK |
| 预测分子量 | 12.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Sprr2b重组蛋白的3篇参考文献示例(注：文献为虚拟示例，实际引用需核实真实来源)：
---
1. **文献名称**: *"Recombinant SPRR2B Protein Enhances Epidermal Barrier Repair in Murine Models"*
**作者**: Zhang L, et al.
**摘要**: 本研究利用大肠杆菌表达系统成功纯化重组Sprr2b蛋白，并证明其在小鼠皮肤损伤模型中能够加速表皮屏障修复，机制可能与调控角蛋白交联相关。
2. **文献名称**: *"Expression and Functional Characterization of SPRR2B in Psoriasis Pathogenesis"*
**作者**: Gupta S, et al.
**摘要**: 通过哺乳动物细胞系表达重组Sprr2b，发现其在银屑病皮损组织中表达上调，体外实验表明Sprr2b通过激活炎症信号通路加剧角质形成细胞过度增殖。
3. **文献名称**: *"Structural Analysis of Recombinant SPRR2B and Its Role in Oxidative Stress Response"*
**作者**: Müller R, et al.
**摘要**: 采用X射线晶体学解析了重组Sprr2b的三维结构，发现其富含半胱氨酸的结构域在氧化应激条件下参与细胞保护作用，为抗氧化治疗提供潜在靶点。
---
**说明**：以上文献为示例性质，实际研究中需通过PubMed、Web of Science等平台检索真实文献。建议结合具体研究方向筛选关键词(如“Sprr2b recombinant expression”、“SPRR2B function”)获取准确信息。
The Small Proline-Rich Protein 2B (Sprr2b) is a member of the Sprr protein family, which comprises cysteine-rich, cross-linking proteins critical for epidermal differentiation and barrier formation. Primarily expressed in stratified squamous epithelia, Sprr2b is localized to the epidermal differentiation complex on chromosome 1q21. It plays a key role in cornified envelope assembly—a specialized structure essential for skin integrity—by cross-linking loricrin, involucrin, and other structural proteins via transglutaminase-mediated isopeptide bonds. This contributes to mechanical resilience and protection against environmental stressors like UV radiation, pathogens, and dehydration.
Sprr2b is upregulated during keratinocyte terminal differentiation and under oxidative stress, suggesting dual roles in tissue repair and stress response. Its expression is regulated by transcription factors (e.g., AP-1. p63) and signaling pathways (e.g., Notch, NF-κB). Dysregulation of Sprr2b has been implicated in skin disorders such as psoriasis, atopic dermatitis, and cancer, where abnormal cornification or proliferation disrupts barrier function.
Recombinant Sprr2b protein, typically produced in bacterial (E. coli) or mammalian expression systems, retains its ability to interact with epidermal proteins, making it a valuable tool for studying skin biology. Researchers use it to model cornified envelope dynamics, screen therapeutic agents for barrier-related diseases, or explore its potential as a biomarker. Its small size (~10-15 kDa), proline-rich repeats, and disulfide bond-dependent folding pose challenges for purification, often requiring refolding steps or fusion tags. Current studies also investigate its extracellular roles in immunomodulation and wound healing, expanding its relevance beyond structural support.
×
