| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Setd4 |
| Uniprot No | Q9NVD3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-307aa |
| 氨基酸序列 | MQKGKGRTSRIRRRKLCGSSESRGVNESHKSEFIELRKWLKARKFQDSNLAPACFPGTGR GLMSQTSLQEGQMIISLPESCLLTTDTVIRSYLGAYITKWKPPPSPLLALCTFLVSEKHA GHRSLWKPYLEILPKAYTCPVCLEPEVVNLLPKSLKAKAEEQRAHVQEFFASSRDFFSSL QPLFAEAVDSIFSYSALLWAWCTVNTRAVYLRPRQRECLSAEPDTCALAPYLDLLNHSPH VQVKAAFNEETHSYEIRTTSRWRKHEEVFICYGPHDNQRLFLEYGFVSVHNPHACVYVSR GWNQLCS |
| 预测分子量 | 50,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于Setd4(SET domain-containing protein 4)重组蛋白的研究目前较为有限，以下为基于领域相关性的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **文献名称**: "SETD4 regulates histone methylation and pluripotency in embryonic stem cells"
**作者**: Zhang Y, et al.
**摘要**: 本研究报道了SETD4重组蛋白在胚胎干细胞中的组蛋白甲基化功能，发现其通过催化H3K36甲基化调控干细胞的自我更新和分化，重组蛋白的体外实验证实了其酶活性依赖性作用。
2. **文献名称**: "Recombinant SETD4 protein expression and purification for structural analysis"
**作者**: Lee S, et al.
**摘要**: 作者优化了SETD4重组蛋白在大肠杆菌中的表达与纯化流程，并通过X射线晶体学解析了其催化结构域的三维结构，为后续功能研究提供基础。
3. **文献名称**: "SETD4 interacts with p53 and modulates DNA damage response"
**作者**: Wang H, et al.
**摘要**: 利用重组SETD4蛋白进行体外结合实验，发现其与肿瘤抑制蛋白p53直接互作，并通过甲基化修饰调节DNA损伤修复通路活性。
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**注意**：以上文献为模拟示例，实际研究中可能需查阅最新数据库(如PubMed、Google Scholar)。目前公开领域对SETD4重组蛋白的深入研究较少，建议结合关键词“SETD4 recombinant protein”或“SETD4 in vitro”进一步检索近期论文。
**Background of SETD4 Recombinant Protein**
SETD4 (SET domain-containing protein 4) is a member of the SET domain-containing methyltransferase family, enzymes known for their role in epigenetic regulation through post-translational modification of histones. Unlike its well-characterized relatives (e.g., SETD1 or SETD7), SETD4 remains less studied, though emerging evidence suggests its involvement in critical cellular processes, including chromatin remodeling, gene expression regulation, and stress response pathways. The protein contains a conserved SET domain, a catalytic module responsible for methyltransferase activity, though its specific histone or non-histone substrates remain under investigation.
Recombinant SETD4 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Its production allows researchers to probe its enzymatic mechanisms, substrate specificity, and interaction partners in vitro. Recent studies hint at SETD4's potential role in DNA damage repair and cellular senescence, with implications in aging-related diseases and cancer. For instance, SETD4 may methylate non-histone proteins to modulate their activity, expanding its functional scope beyond classical epigenetic roles.
The development of SETD4 recombinant protein has also facilitated drug discovery efforts, particularly in designing inhibitors to target aberrant methyltransferase activity in diseases. However, challenges persist in clarifying its physiological targets and regulatory networks. Ongoing research aims to unravel its contribution to developmental biology, stem cell maintenance, and immune responses, positioning SETD4 as a protein of growing interest in both basic and translational biomedical research.
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