| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMEM168 |
| Uniprot No | Q9H0V1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-697aa |
| 氨基酸序列 | MCKSLRYCFSHCLYLAMTRLEEVNREVNMHSSVRYLGYLARINLLVAICLGLYVRWEKTANSLILVIFILGLFVLGIASILYYYFSMEAASLSLSNLWFGFLLGLLCFLDNSSFKNDVKEESTKYLLLTSIVLRILCSLVERISGYVRHRPTLLTTVEFLELVGFAIASTTMLVEKSLSVILLVVALAMLIIDLRMKSFLAIPNLVIFAVLLFFSSLETPKNPIAFACFFICLITDPFLDIYFSGLSVTERWKPFLYRGRICRRLSVVFAGMIELTFFILSAFKLRDTHLWYFVIPGFSIFGIFWMICHIIFLLTLWGFHTKLNDCHKVYFTHRTDYNSLDRIMASKGMRHFCLISEQLVFFSLLATAILGAVSWQPTNGIFLSMFLIVLPLESMAHGLFHELGNCLGGTSVGYAIVIPTNFCSPDGQPTLLPPEHVQELNLRSTGMLNAIQRFFAYHMIETYGCDYSTSGLSFDTLHSKLKAFLELRTVDGPRHDTYILYYSGHTHGTGEWALAGGDTLRLDTLIEWWREKNGSFCSRLIIVLDSENSTPWVKEVRKINDQYIAVQGAELIKTVDIEEADPPQLGDFTKDWVEYNCNSSNNICWTEKGRTVKAVYGVSKRWSDYTLHLPTGSDVAKHWMLHFPRITYPLVHLANWLCGLNLFWICKTCFRCLKRLKMSWFLPTVLDTGQGFKLVKS |
| 预测分子量 | 79,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TMEM168重组蛋白的虚构参考文献示例(仅供格式参考，实际文献需通过学术数据库查询)：
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1. **文献名称**: *TMEM168重组蛋白调控伏隔核神经元兴奋性的机制研究*
**作者**: Yamada S, et al.
**摘要**: 本研究通过在大鼠原代神经元中表达TMEM168重组蛋白，发现其通过调节电压门控钠通道活性，增强伏隔核神经元的兴奋性，可能参与甲基苯丙胺成瘾行为的分子机制。
2. **文献名称**: *TMEM168重组蛋白与β-淀粉样蛋白相互作用的体外验证*
**作者**: Chen L, et al.
**摘要**: 利用重组TMEM168蛋白进行体外结合实验，证实其直接结合阿尔茨海默病相关β-淀粉样蛋白(Aβ1-42)，并抑制Aβ纤维化，提示其在神经退行性疾病中的潜在保护作用。
3. **文献名称**: *TMEM168重组蛋白的晶体结构解析及其功能域分析*
**作者**: Smith J, et al.
**摘要**: 通过X射线晶体学首次解析TMEM168重组蛋白的3D结构，揭示其C端跨膜结构域的关键作用，为设计靶向TMEM168的小分子药物提供结构基础。
4. **文献名称**: *肝脏特异性敲除TMEM168对脂代谢的影响及重组蛋白回补实验*
**作者**: Wang H, et al.
**摘要**: 在TMEM168肝脏特异性敲除小鼠模型中，发现脂质代谢异常，而通过腺病毒递送重组TMEM168蛋白可逆转表型，表明其在肝脏脂质稳态中的必要性。
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**注意**：以上为模拟示例，实际文献需通过PubMed、Web of Science等平台以“TMEM168 recombinant protein”等关键词检索。如需具体文献，建议提供更详细的研究方向(如疾病模型、分子机制等)。
The TMEM168 (Transmembrane Protein 168) recombinant protein is engineered to study the functional and structural roles of the native TMEM168 protein, which belongs to the transmembrane protein family. TMEM168 has garnered attention for its potential involvement in neurological processes, including neural signaling and addiction-related behaviors. Studies suggest it may interact with voltage-gated calcium channels (e.g., CaV2.1) and influence neurotransmitter release, synaptic plasticity, and neuroadaptation mechanisms, particularly in contexts like cocaine addiction. Its recombinant form is typically produced using expression systems (e.g., E. coli, mammalian cells) to enable in vitro investigations, offering controlled purity and specificity for biochemical assays.
Structurally, TMEM168 contains multiple transmembrane domains, with intracellular N- and C-terminal regions that may participate in protein-protein interactions. The recombinant protein often retains these features, allowing researchers to explore its topology, post-translational modifications, and binding partners. Its involvement in regulating calcium channel activity has linked it to pathways affecting neuronal excitability and reward circuits, making it a target for neuropsychiatric disorder research.
Current applications of TMEM168 recombinant protein include mechanistic studies of addiction, screening for therapeutic compounds, and antibody production. Challenges remain in fully elucidating its physiological roles, but recombinant tools provide a foundation for mapping its interactions and validating its relevance in disease models. Ongoing research aims to clarify its contributions to neuroadaptation and its potential as a biomarker or drug target.
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