| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Col26a1 |
| Uniprot No | Q96A83 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-441aa |
| 氨基酸序列 | TGFLYPFSAA ALQQHGYPEP GAGSPGSGYA SRRHWCHHTV TRTVSCQVQN GSETVVQRVY QSCRWPGPCA NLVSYRTLIR PTYRVSYRTV TVLEWRCCPG FTGSNCDEEC MNCTRLSDMS ERLTTLEAKV LLLEAAERPS SPDNDLPAPE STPPTWNEDF LPDAIPLAHP VPRQRRPTGP AGPPGQTGPP GPAGPPGSKG DRGQTGEKGP AGPPGLLGPP GPRGLPGEMG RPGPPGPPGP AGNPGPSPNS PQGALYSLQP PTDKDNGDSR LASAIVDTVL AGVPGPRGPP GPPGPPGPRG PPGPPGTPGS QGLAGERGTV GPSGEPGVKG EEGEKAATAE GEGVQQLREA LKILAERVLI LEHMIGIHDP LASPEGGSGQ DAALRANLKM KRGGAQPDGV LAALLGPDPG QKSVDQASSR K |
| 预测分子量 | 45,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COL26A1重组蛋白的3篇参考文献，信息基于公开研究整理：
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1. **文献名称**: *"Recombinant expression and structural characterization of collagen XXVI α1 chain in vertebrate development"*
**作者**: Smith A, et al.
**摘要**: 研究通过重组技术在大肠杆菌中表达COL26A1蛋白，分析了其结构中的胶原三螺旋域和非胶原区的功能，并发现其在斑马鱼胚胎发育中参与肌肉-肌腱连接的形成。
2. **文献名称**: *"Production and functional analysis of COL26A1 in extracellular matrix remodeling"*
**作者**: Li H, et al.
**摘要**: 利用哺乳动物细胞系统(HEK293)重组表达COL26A1.证实其可通过整合素信号通路调控细胞黏附和迁移，并参与伤口愈合过程中的基质重塑。
3. **文献名称**: *"COL26A1 as a novel biomarker in fibrosis: Recombinant protein-based mechanistic insights"*
**作者**: Tanaka K, et al.
**摘要**: 通过昆虫细胞表达系统制备重组COL26A1.发现其在小鼠肝纤维化模型中异常上调，并通过TGF-β通路促进成纤维细胞活化，提示其作为纤维化治疗靶点的潜力。
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**注**：以上文献为示例性内容，实际研究中COL26A1相关重组蛋白的文献较少，建议通过PubMed或SciFinder以关键词“COL26A1 recombinant”检索最新论文。如需具体文章，可进一步提供研究方向(如表达系统、疾病模型等)。
**Background of COL26A1 Recombinant Protein**
Collagen type XXVI alpha 1 (COL26A1) is a lesser-studied member of the collagen family, a group of structural proteins critical for maintaining tissue integrity and cellular signaling. Unlike classical fibrillar collagens (e.g., types I, II, III), COL26A1 belongs to the "non-fibrillar" subclass, characterized by unique domain structures and tissue-specific expression patterns. It contains a short collagenous domain flanked by non-collagenous regions, which may mediate interactions with other extracellular matrix (ECM) components or cell surface receptors.
COL26A1 is spatiotemporally regulated, with prominent expression reported during embryonic development, particularly in skeletal and smooth muscle tissues, suggesting roles in morphogenesis. In adults, its expression becomes restricted, detected in tissues like the testes, ovaries, and certain epithelial cells. Functional studies remain limited, but COL26A1 is hypothesized to influence cell adhesion, migration, and tissue remodeling. Notably, aberrant COL26A1 expression has been linked to pathological conditions, including fibrosis and cancer progression, where it may modulate ECM stiffness or tumor microenvironment signaling.
Recombinant COL26A1 protein is typically produced in mammalian expression systems to ensure proper post-translational modifications (e.g., hydroxylation of proline residues). This engineered protein enables in vitro studies to dissect its structural motifs, binding partners, and mechanistic roles. Researchers utilize it to generate antibodies, map interaction networks, or model COL26A1-associated diseases. Challenges include its low natural abundance and poorly understood regulation, which complicate functional characterization. Nevertheless, recombinant COL26A1 serves as a vital tool for unraveling its contributions to ECM dynamics and potential therapeutic applications in connective tissue disorders or cancer.
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