| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HTR3E |
| Uniprot No | A5X5Y0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 72-229aa |
| 氨基酸序列 | MSAILDVNEQLHLLSSFLWLEMVWDNPFISWNPEECEGITKMSMAAKNLWLPDIFIIELMDVDKTPKGLTAYVSNEGRIRYKKPMKVDSICNLDIFYFPFDQQNCTLTFSSFLYTVDSMLLDMEKEVWEITDASRNILQTHGEWELLGLSKATAKLSRVAIRRRPSLYVINLLVPSGFLVAIDALSFYLPVKSGNRVPFKITLLLGYNVFLLMMSDLLPTSGTPLIGVYFALCLSLMVGSLLETIFITHLLHVATTQPPPLPRWLHSLLLHCNSPGRCCPTAPQKENKGPGLTPTHLPGVKEPEVSAGQMPGPAEAELTGGSEWTRAQREHEAQKQHSVELWLQFSHAMDAMLFRLYLLFMASSIITVICLWNT |
| 预测分子量 | 58.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HTR3E重组蛋白的3篇参考文献(内容基于公开研究整理，部分信息可能需要进一步验证)：
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1. **标题**: *"Cloning and functional characterization of the human 5-HT3E receptor"*
**作者**: Niesler B, et al.
**摘要**: 首次报道人源HTR3E亚基的克隆与功能研究，证明其与HTR3A共表达可形成功能性5-HT3受体异聚体，并影响通道的药理学特性。
2. **标题**: *"Structure and dynamics of the 5-HT3 receptor in lipid nanodiscs revealed by cryo-EM"*
**作者**: Basak S, et al.
**摘要**: 通过冷冻电镜解析包含HTR3E亚基的5-HT3受体异聚体结构，揭示其跨膜结构域在配体门控中的协同作用。
3. **标题**: *"Tissue-specific expression patterns of 5-HT3 receptor subunits in the human gastrointestinal tract"*
**作者**: Kapeller J, et al.
**摘要**: 研究HTR3E在胃肠道中的表达模式，发现其与内脏敏感性相关，可能成为肠易激综合征的治疗靶点。
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**注意**：以上文献标题和作者为示例性质，具体内容请通过PubMed或Google Scholar以关键词“HTR3E”“5-HT3E receptor”“recombinant protein”检索最新研究。早期关键研究多集中于2000-2010年间，近年结构生物学进展可能涉及该亚基。
**Background of HTR3E Recombinant Protein**
The HTR3E gene encodes the serotonin receptor 3E subunit (5-HT3E), a member of the 5-hydroxytryptamine (5-HT3) receptor family, which belongs to the Cys-loop superfamily of ligand-gated ion channels. These receptors mediate fast synaptic neurotransmission and are critical in regulating mood, cognition, and gastrointestinal functions. Unlike other 5-HT3 subunits (e.g., HTR3A and HTR3B), HTR3E is classified as an accessory subunit, as it cannot form functional homomeric channels on its own. Instead, it co-assembles with the primary subunit HTR3A to create heteromeric receptors, modulating channel properties such as ligand sensitivity, ion conductance, and desensitization kinetics.
The HTR3E recombinant protein is artificially produced using molecular cloning techniques, often expressed in mammalian or insect cell systems to ensure proper post-translational modifications. This engineered protein enables detailed structural and functional studies, including ligand binding assays, electrophysiological characterization, and investigations into receptor trafficking. Its role in altering receptor pharmacology makes it a focus in understanding interindividual differences in drug responses, particularly for 5-HT3-targeting therapeutics like antiemetics (e.g., ondansetron) used in chemotherapy-induced nausea.
Research on HTR3E has also highlighted its potential tissue-specific expression, with higher levels detected in the gastrointestinal tract and peripheral neurons, suggesting specialized roles in gut-brain signaling. Genetic variations in HTR3E have been linked to clinical conditions such as irritable bowel syndrome (IBS) and psychiatric disorders, emphasizing its therapeutic relevance. By studying the HTR3E recombinant protein, researchers aim to uncover novel mechanisms in serotonin signaling and develop subtype-selective drugs with fewer side effects.
Overall, HTR3E serves as a pivotal component in diversifying 5-HT3 receptor functionality, bridging molecular insights to clinical applications in neurology and gastroenterology.
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