| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EDAR |
| Uniprot No | Q9UNE0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-448aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSEYSNCGENEYYNQTTGLCQECPPCGPG EEPYLSCGYGTKDEDYGCVPCPAEKFSKGGYQICRRHKDCEGFFRATVLT PGDMENDAECGPCLPGYYMLENRPRNIYGMVCYSCLLAPPNTKECVGATS GASANFPGTSGSSTLSPFQHAHKELSGQGHLATALIIAMSTIFIMAIAIV LIIMFYILKTKPSAPACCTSHPGKSVEAQVSKDEEKKEAPDNVVMFSEKD EFEKLTATPAKPTKSENDASSENEQLLSRSVDSDEEPAPDKQGSPELCLL SLVHLAREKSATSNKSAGIQSRRKKILDVYANVCGVVEGLSPTELPFDCL EKTSRMLSSTYNSEKAVVKTWRHLAESFGLKRDEIGGMTDGMQLFDRIST AGYSIPELLTKLVQIERLDAVESLCADILEWAGVVPPASQPHAAS |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EDAR重组蛋白的3篇代表性文献摘要及作者信息(注：文献为示例性质，具体内容需通过学术数据库核实)：
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1. **文献名称**: *"Structural and functional analysis of EDAR reveals molecular mechanisms of ectodermal dysplasia"*
**作者**: Zhang Y, et al.
**摘要**: 本研究解析了重组EDAR蛋白的晶体结构，揭示了其胞外结构域与配体EDA的相互作用机制，并发现特定突变通过破坏受体二聚化导致外胚层发育不良的表型。
2. **文献名称**: *"Recombinant EDAR protein activates NF-κB signaling in vitro and rescues tabby mouse phenotypes in vivo"*
**作者**: Cui CY, Schlessinger D.
**摘要**: 通过表达重组EDAR蛋白，研究证实其可激活下游NF-κB信号通路，并在Tabby小鼠模型中成功恢复毛发和汗腺发育缺陷，为治疗X连锁外胚层发育不良提供实验依据。
3. **文献名称**: *"EDAR recombinant ectodomain binds EDA with high affinity and modulates keratinocyte differentiation"*
**作者**: Mikkola ML, Thesleff I.
**摘要**: 利用重组EDAR胞外域蛋白进行体外结合实验，发现其与EDA配体具有纳摩尔级亲和力，并证明该互作可调控表皮角质形成细胞的分化方向。
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**建议**：以上文献为模拟示例，实际引用时请通过PubMed或Google Scholar检索关键词“EDAR recombinant protein”或结合具体研究方向筛选近年高引论文。
**Background of EDAR Recombinant Protein**
The Ectodysplasin A receptor (EDAR) is a transmembrane protein belonging to the tumor necrosis factor receptor (TNFR) superfamily. It plays a critical role in embryonic development, particularly in the formation of ectodermal tissues such as hair follicles, teeth, sweat glands, and mammary glands. EDAR is activated by its ligand, ectodysplasin-A (EDA), initiating downstream signaling cascades, including the NF-κB pathway, which regulates cell differentiation, survival, and tissue morphogenesis. Mutations in the *EDAR* gene are linked to ectodermal dysplasias, a group of genetic disorders characterized by abnormal development of skin, hair, teeth, and glands.
Recombinant EDAR protein is engineered in vitro using expression systems like mammalian cells, bacteria, or yeast to produce soluble, functional forms of the receptor or its domains. This protein retains the ability to bind EDA, enabling researchers to study EDAR-EDA interactions, signaling mechanisms, and their roles in developmental biology and disease. Its applications extend to structural studies, drug screening, and therapeutic development. For example, recombinant EDAR has been explored in preclinical studies to rescue ectodermal defects in animal models of EDA-deficient disorders.
Additionally, EDAR recombinant protein serves as a tool to investigate evolutionary adaptations. Certain *EDAR* variants, like the 370A allele prevalent in East Asian populations, are associated with thickened hair and altered sweat gland density, highlighting its role in human adaptation to diverse environments. By leveraging recombinant EDAR, scientists aim to decode its functional impact on cellular pathways and develop targeted therapies for ectodermal dysplasias or inflammatory conditions linked to TNFR signaling dysregulation. Overall, EDAR recombinant protein bridges basic research and translational medicine, offering insights into developmental biology, genetic diseases, and evolutionary genetics.
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