| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCC1 |
| Uniprot No | P33527 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1248-1531aa |
| 氨基酸序列 | VRMSSEMETNIVAVERLKEYSETEKEAPWQIQETAPPSSWPQVGRVEFRNYCLRYREDLDFVLRHINVTINGGEKVGIVGRTGAGKSSLTLGLFRINESAEGEIIIDGINIAKIGLHDLRFKITIIPQDPVLFSGSLRMNLDPFSQYSDEEVWTSLELAHLKDFVSALPDKLDHECAEGGENLSVGQRQLVCLARALLRKTKILVLDEATAAVDLETDDLIQSTIRTQFEDCTVLTIAHRLNTIMDYTRVIVLDKGEIQEYGAPSDLLQQRGLFYSMA kDaGLV |
| 预测分子量 | 35.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCC1(MRP1)重组蛋白研究的模拟参考文献示例(仅供格式参考，具体文献需通过学术数据库检索)：
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1. **标题**: "Functional characterization of recombinant human ABCC1/MRP1 expressed in insect cells"
**作者**: Deeley RG, et al.
**摘要**: 本研究利用杆状病毒-昆虫细胞系统重组表达了人ABCC1蛋白，证实其具有ATP依赖性的药物外排活性，并揭示了其底物特异性与肿瘤多药耐药性的关联。
2. **标题**: "Structural analysis of the nucleotide-binding domains of ABCC1 through recombinant protein mutagenesis"
**作者**: Leslie EM, et al.
**摘要**: 通过重组蛋白定点突变技术，解析ABCC1的核苷酸结合域(NBD)构象变化，阐明ATP水解与底物转运的分子机制。
3. **标题**: "Reconstitution of ABCC1-mediated drug transport in proteoliposomes"
**作者**: Rius M, et al.
**摘要**: 将重组纯化的ABCC1蛋白嵌入脂质体，建立体外转运模型，定量分析其对抗癌药物(如长春新碱)的转运效率及抑制剂敏感性。
4. **标题**: "Role of N-linked glycosylation in the trafficking and function of recombinant ABCC1"
**作者**: Conseil G, et al.
**摘要**: 通过哺乳动物细胞表达系统，研究糖基化修饰对ABCC1蛋白细胞膜定位及转运活性的调控作用，发现特定糖基化位点缺失影响其稳定性。
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**注意**：以上为示例性内容，实际文献需通过PubMed、Web of Science等平台以“ABCC1/MRP1 recombinant protein”为关键词检索获取。
ABCC1 (ATP-binding cassette subfamily C member 1), also known as multidrug resistance-associated protein 1 (MRP1), is a member of the ABC transporter superfamily, which plays a critical role in cellular detoxification and drug resistance. This 190 kDa transmembrane protein is encoded by the ABCC1 gene in humans and is ubiquitously expressed in tissues, particularly in the lung, testis, and immune cells. Structurally, ABCC1 consists of two membrane-spanning domains (MSDs) and two nucleotide-binding domains (NBDs), enabling ATP-dependent transport of substrates across cell membranes.
ABCC1 primarily functions as an efflux pump for organic anions, conjugates (e.g., glutathione-, glucuronide-, or sulfate-conjugated metabolites), and xenobiotics, including chemotherapeutic agents like vincristine, doxorubicin, and methotrexate. Its ability to export these compounds contributes to multidrug resistance (MDR) in cancer cells, a major challenge in oncology. Beyond drug resistance, ABCC1 participates in physiological processes such as leukotriene C4-mediated inflammatory responses, redox balance via glutathione transport, and cellular signaling.
Recombinant ABCC1 protein is produced using expression systems (e.g., insect or mammalian cells) to study its structure-function relationships, substrate specificity, and interactions with inhibitors. Purified recombinant ABCC1 enables in vitro assays to screen modulators that could overcome MDR or regulate inflammation. Research on ABCC1 also extends to neurodegenerative and cardiovascular diseases, where its dysregulation impacts oxidative stress and toxin clearance. As a therapeutic target, understanding ABCC1’s molecular mechanisms remains vital for developing strategies to counteract its role in pathological conditions.
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