| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLINT1 |
| Uniprot No | Q14677 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-625aa |
| 氨基酸序列 | MLNMWKVRELVDKATNVVMNYSEIESKVREATNDDPWGPSGQLMGEIAKATFMYEQFPELMNMLWSRMLKDNKKNWRRVYKSLLLLAYLIRNGSERVVTSAREHIYDLRSLENYHFVDEHGKDQGINIRQKVKELVEFAQDDDRLREERKKAKKNKDKYVGVSSDSVGGFRYSERYDPEPKSKWDEEWDKNKSAFPFSDKLGELSDKIGSTIDDTISKFRRKDREDSPERCSDSDEEKKARRGRSPKGEFKDEEETVTTKHIHITQATETTTTRHKRTANPSKTIDLGAAAHYTGDKASPDQNASTHTPQSSVKTSVPSSKSSGDLVDLFDGTSQSTGGSADLFGGFADFGSAAASGSFPSQVTATSGNGDFGDWSAFNQAPSGPVASSGEFFGSASQPAVELVSGSQSALGPPPAASNSSDLFDLMGSSQATMTSSQSMNFSMMSTNTVGLGLPMSRSQNTDMVQKSVSKTLPSTWSDPSVNISLDNLLPGMQPSKPQQPSLNTMIQQQNMQQPMNVMTQSFGAVNLSSPSNMLPVRPQTNALIGGPMPMSMPNVMTGTMGMAPLGNTPMMNQSMMGMNMNIGMSAAGMGLTGTMGMGMPNIAMTSGTVQPKQDAFANFANFSK |
| 预测分子量 | 75.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟的3-4篇关于CLINT1重组蛋白的参考文献示例(注：文献为假设性构造，实际研究中请通过学术数据库验证)：
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1. **标题**: *Structural and Functional Analysis of CLINT1 in Clathrin-Mediated Endocytosis*
**作者**: Müller, A. et al.
**摘要**: 本研究通过大肠杆菌表达系统纯化重组CLINT1蛋白，结合X射线晶体学解析其ENTH结构域的三维结构，揭示了其与磷脂酰肌醇4.5-二磷酸(PIP2)的特异性结合机制，并证实该相互作用对网格蛋白包被囊泡的形成至关重要。
2. **标题**: *Recombinant CLINT1 Facilitates Synaptic Vesicle Recycling in Vitro*
**作者**: Tanaka, K. & Park, S.
**摘要**: 利用昆虫细胞表达体系获得高纯度CLINT1重组蛋白，体外实验表明其通过促进网格蛋白与AP2衔接蛋白的互作，增强突触囊泡的再循环效率，为神经递质释放机制提供了新见解。
3. **标题**: *Optimization of CLINT1 Recombinant Protein Production for Drug Screening*
**作者**: Gupta, R. et al.
**摘要**: 开发了一种基于哺乳动物细胞的重组CLINT1表达及纯化方案，验证其在基于表面等离子体共振(SPR)的药物筛选平台中的应用，成功鉴定出多个潜在的小分子抑制剂。
4. **标题**: *CLINT1-EGFR Interaction Dynamics Studied via Recombinant Protein Pull-Down Assays*
**作者**: Li, X. & Wang, H.
**摘要**: 通过构建His标签重组CLINT1蛋白，进行下拉实验与质谱分析，首次报道CLINT1与表皮生长因子受体(EGFR)的直接相互作用，提示其在癌症信号通路中的潜在调控作用。
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**提示**：实际研究中，建议通过PubMed、Google Scholar等平台以关键词“CLINT1 recombinant”“CLINT1 purification”或“CLINT1 structure/function”检索最新文献，并优先选择高影响力期刊文章(如*Journal of Cell Biology*、*Traffic*等)。
**Background of CLINT1 Recombinant Protein**
CLINT1 (Clathrin Interactor 1), also known as ENT domain-containing protein 1 (ENTHD1), is a multifunctional adaptor protein involved in intracellular trafficking, particularly clathrin-mediated endocytosis (CME). It interacts with clathrin, AP-2 complexes, and other endocytic machinery components, facilitating vesicle formation and cargo sorting. CLINT1 contains an N-terminal ENTH (epsin N-terminal homology) domain, which binds phosphatidylinositol lipids, and a C-terminal disordered region that mediates protein-protein interactions. These structural features enable CLINT1 to act as a scaffold, coordinating membrane curvature and cargo recruitment during endocytosis.
Recombinant CLINT1 protein is engineered to study its biochemical properties, interactions, and role in cellular processes. Produced using heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains functional domains for binding partners like clathrin, ubiquitin, or synaptic vesicle proteins. Researchers utilize CLINT1 recombinant proteins to dissect endocytic pathways, investigate membrane trafficking defects, or explore its involvement in neurological disorders. For instance, CLINT1 dysfunction has been linked to impaired synaptic vesicle recycling, potentially contributing to neurodegenerative diseases such as Alzheimer’s or epilepsy.
Additionally, CLINT1’s role in regulating receptor internalization (e.g., EGFR, GPCRs) makes it relevant in cancer and signal transduction studies. Recombinant variants (e.g., truncated forms or tagged versions) enable structure-function analyses, antibody development, and high-throughput screening for therapeutic targets. Its applications extend to *in vitro* reconstitution assays, elucidating molecular mechanisms of vesicle formation and cargo sorting. Overall, CLINT1 recombinant proteins serve as critical tools for advancing understanding of endocytosis and its implications in health and disease.
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