| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT4 |
| Uniprot No | P19013 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-534aa |
| 氨基酸序列 | MIARQQCVRGGPRGFSCGSAIVGGGKRGAFSSVSMSGGAGRCSSGGFGSRSLYNLRGNKSISMSVAGSRQGACFGGAGGFGTGGFGAGGFGAGFGTGGFGGGFGGSFSGKGGPGFPVCPAGGIQEVTINQSLLTPLHVEIDPEIQKVRTEEREQIKLLNNKFASFIDKVQFLEQQNKVLETKWNLLQQQTTTTSSKNLEPLFETYLSVLRKQLDTLGNDKGRLQSELKTMQDSVEDFKTKYEEEINKRTAAENDFVVLKKDVDAAYLNKVELEAKVDSLNDEINFLKVLYDAELSQMQTHVSDTSVVLSMDNNRNLDLDSIIAEVRAQYEEIAQRSKAEAEALYQTKVQQLQISVDQHGDNLKNTKSEIAELNRMIQRLRAEIENIKKQCQTLQVSVADAEQRGENALKDAHSKRVELEAALQQAKEELARMLREYQELMSVKLALDIEIATYRKLLEGEEYRMSGECQSAVSISVVSGSTSTGGISGGLGSGSGFGLSSGFGSGSGSGFGFGGSVSGSSSSKIISTTTLNKRR |
| 预测分子量 | 59.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT4重组蛋白的3篇示例参考文献(内容基于学术研究常见方向虚构，仅供参考)：
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1. **文献名称**：*"Expression and Purification of Recombinant Human Keratin 4 (KRT4) in a Bacterial System"*
**作者**：Zhang, L. et al.
**摘要**：本研究通过在大肠杆菌中表达带有His标签的重组KRT4蛋白，优化了诱导条件并使用镍柱亲和层析纯化，验证了其与天然KRT4的免疫反应性，为后续功能研究提供高纯度蛋白。
2. **文献名称**：*"Structural Characterization of KRT4 Recombinant Protein and Its Role in Epithelial Integrity"*
**作者**：Lee, S. et al.
**摘要**：利用圆二色谱和电子显微镜分析重组KRT4的二级结构和纤维组装能力，发现其通过维持上皮细胞骨架稳定性参与口腔黏膜屏障功能，为黏膜疾病机制提供依据。
3. **文献名称**：*"Recombinant KRT4 as a Potential Biomarker in Oral Squamous Cell Carcinoma"*
**作者**：Wang, Y. et al.
**摘要**：通过ELISA检测患者血清中重组KRT4水平，发现其显著低于健康对照组，提示KRT4表达缺失可能与口腔癌进展相关，具有临床诊断潜力。
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**备注**：若需真实文献，建议通过PubMed或Google Scholar检索关键词“KRT4 recombinant protein”以获取最新研究。部分相关领域可能涉及KRT4突变分析或疾病模型构建。
Keratin 4 (KRT4), a member of the type II keratin family, is a structural protein critical for maintaining the integrity of epithelial tissues. As part of the intermediate filament network, KRT4 pairs with type I keratins (e.g., KRT13) to form heterodimers that provide mechanical stability and resilience to epithelial cells. It is predominantly expressed in stratified squamous epithelia, including the oral mucosa, esophagus, and vaginal epithelium, where it supports cellular differentiation and tissue-specific functions. Mutations in the KRT4 gene are linked to autosomal dominant disorders like white sponge nevus, characterized by abnormal epithelial thickening and fragility, underscoring its biological importance.
Recombinant KRT4 protein is produced using biotechnological platforms such as bacterial (E. coli) or mammalian expression systems. These systems enable large-scale production of purified, functional KRT4 for research and therapeutic applications. The recombinant protein typically retains post-translational modifications when expressed in mammalian cells, mimicking native keratin behavior. Advanced purification techniques, including affinity chromatography with tags like His-tag, ensure high purity and specificity.
In research, recombinant KRT4 serves as a tool to study epithelial pathophysiology, cell signaling, and protein-protein interactions. It aids in developing disease models, screening therapeutic compounds, and generating antibodies for diagnostic assays. Additionally, it has potential in tissue engineering to mimic epithelial microenvironments for regenerative medicine. By elucidating KRT4's role in disease mechanisms and epithelial dynamics, recombinant KRT4 contributes to advancements in dermatology, oncology, and genetic disorder research.
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