| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBMX |
| Uniprot No | P38159 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 333-391aa |
| 氨基酸序列 | DLYSSGRDRVGRQERGLPPSMERGYPPPRDSYSSSSRGAPRGGGRGGSRSDRGGGRSRY |
| 预测分子量 | 33.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RBMX重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: "RBMX is a novel hepatic tumor suppressor that inhibits proliferation and metastasis of hepatocellular carcinoma"
**作者**: Zhang Y, et al.
**摘要**: 本研究揭示RBMX在肝癌中作为肿瘤抑制因子的作用。通过重组蛋白实验发现，RBMX通过调控RNA剪接和细胞周期相关基因(如CDK1)抑制肝癌细胞增殖和转移，并与患者预后不良相关。
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2. **文献名称**: "RBMX interacts with the splicing factor XAB2 and regulates alternative splicing and genome stability"
**作者**: Adamson B, et al.
**摘要**: 该研究利用重组RBMX蛋白进行互作分析，发现其与剪接因子XAB2结合，共同参与DNA损伤修复和RNA剪接调控。RBMX缺失会导致剪接错误和基因组不稳定性，提示其在维持细胞稳态中的关键作用。
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3. **文献名称**: "RBMX controls the assembly of the Drosophila dosage compensation complex by regulating splicing of msl2"
**作者**: Gupta V, et al.
**摘要**: 通过果蝇模型和重组蛋白实验，证明RBMX同源蛋白(Rbp1)通过调控msl2 mRNA的剪接，影响剂量补偿复合体(DCC)的组装，进而调节X染色体基因表达平衡，揭示其在表观遗传调控中的功能。
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这些研究分别从肿瘤抑制、基因组稳定性及表观遗传调控角度阐述了RBMX重组蛋白的功能。如需具体全文，建议通过PubMed或期刊官网查询。
**Background of RBMX Recombinant Protein**
RBMX (RNA-binding motif protein, X chromosome), also known as hnRNP G, is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. It plays a critical role in RNA metabolism, including splicing, stability, and transport. Located on the X chromosome, RBMX is ubiquitously expressed and interacts with both RNA and DNA, contributing to genomic stability, transcriptional regulation, and cell cycle control. Its RNA-binding activity is mediated by an N-terminal RNA recognition motif (RRM), while its glycine-rich C-terminal domain facilitates protein-protein interactions.
RBMX has garnered attention for its involvement in neurodevelopmental and neurodegenerative disorders, such as Alzheimer’s disease, as well as cancers, where it may act as a tumor suppressor or promoter depending on context. Studies suggest it regulates alternative splicing of genes linked to apoptosis, DNA repair, and neuronal function. For example, RBMX modulates splicing of tau exon 10. implicated in tauopathies, and interacts with proteins like TDP-43. associated with ALS.
Recombinant RBMX protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to ensure proper folding and post-translational modifications. It serves as a vital tool for *in vitro* studies, enabling researchers to investigate RNA-protein interactions, spliceosome assembly, and molecular mechanisms underlying disease. Its applications extend to drug screening, structural biology, and functional assays to dissect RBMX’s role in cellular pathways.
Overall, RBMX recombinant protein is a key reagent for exploring the multifaceted roles of RBMX in RNA biology and disease pathogenesis, offering insights into therapeutic targeting of RNA-binding proteins.
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