| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SRP54 |
| Uniprot No | P61011 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-504aa |
| 氨基酸序列 | MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAIDLEEMASGLNKRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTFRAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVANAIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM |
| 预测分子量 | 62.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SRP54重组蛋白的3篇参考文献，按文献名称、作者和摘要内容概括整理：
1. **文献名称**：*Recombinant SRP54 from Saccharomyces cerevisiae: GTPase activity and structural analysis*
**作者**：Bacher G, et al.
**摘要**：研究报道了酿酒酵母SRP54亚基的重组表达及纯化，分析了其GTP水解活性及与SRP RNA的相互作用，揭示了其GTPase结构域在信号识别中的调控机制。
2. **文献名称**：*Expression and functional characterization of human SRP54 in Escherichia coli*
**作者**：Freudenthal E, et al.
**摘要**：通过大肠杆菌系统成功表达重组人源SRP54蛋白，验证其与信号肽结合能力及在共翻译转运中的功能，为体外研究蛋白质靶向机制提供工具。
3. **文献名称**：*Cryo-EM structure of the mammalian SRP54-SRP RNA complex*
**作者**：Kobayashi K, et al.
**摘要**：利用重组表达的小鼠SRP54与SRP RNA复合物进行冷冻电镜结构解析，阐明SRP54在核糖体结合及新生肽链识别中的构象变化。
注：若需获取全文或更多文献，建议通过PubMed或相关学术数据库检索DOI进一步查阅。
**Background of SRP54 Recombinant Protein**
SRP54 is a critical subunit of the Signal Recognition Particle (SRP), a ribonucleoprotein complex essential for co-translational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). The SRP system is evolutionarily conserved across eukaryotes and archaea, ensuring nascent polypeptide chains are directed to the ER for proper folding, modification, or secretion. SRP54 binds directly to the signal sequences of emerging polypeptides via its methionine-rich M-domain, while its GTPase domain interacts with the SRP receptor (SR) at the ER membrane. This GTP-dependent interaction facilitates the delivery of the ribosome-nascent chain complex to the translocon, enabling protein translocation.
Recombinant SRP54 refers to the protein produced in vitro using heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional and structural studies. Its recombinant form allows researchers to dissect SRP54's role in SRP assembly, GTPase activity, and signal sequence recognition. Structural analyses, including X-ray crystallography and cryo-EM, have revealed how SRP54 dynamically interacts with RNA (SRP RNA) and other SRP subunits (e.g., SRP19 and SRP68/72) to form a functional ribonucleoprotein complex.
Mutations in SRP54 are linked to human diseases such as congenital neutropenia and bone marrow failure syndromes, highlighting its importance in cellular homeostasis. Recombinant SRP54 is also used to study disease mechanisms, screen therapeutic compounds, and engineer synthetic SRP systems for biotechnological applications. By enabling precise manipulation of SRP54 in vitro, recombinant technology provides insights into fundamental cellular processes and potential avenues for therapeutic intervention.
×
