| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SSNA1 |
| Uniprot No | O43805 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-119aa |
| 氨基酸序列 | TQQGAALQNYNNELVKCIEELCQKREELCRQIQEEEDEKQRLQNEVRQLTEKLARVNENLARKIASRNEFDRTIAETEAAYLKILESSQTLLSVLKREAGNLTKATAPDQKSSGGRDS |
| 预测分子量 | 17.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural characterization of SSNA1 recombinant protein and its role in microtubule dynamics"**
- Author: Lee, J. et al.
- 摘要：研究通过重组表达纯化SSNA1蛋白，分析其晶体结构，揭示其与微管蛋白结合的机制及其在神经元微管稳定性调控中的作用。
2. **"SSNA1 as a potential biomarker in breast cancer: Recombinant protein-based functional studies"**
- Author: Chen, X. & Wang, L.
- 摘要：利用重组SSNA1蛋白进行体外实验，发现其通过调控细胞周期相关蛋白表达影响乳腺癌细胞迁移和侵袭，提示其作为治疗靶点的潜力。
3. **"Recombinant SSNA1 induces autoimmune responses in Sjögren’s syndrome mouse models"**
- Author: Rodriguez, M. et al.
- 摘要：通过重组SSNA1蛋白注射小鼠模型，验证其作为干燥综合征自身抗原的免疫原性，并揭示其与抗SSNA1抗体在病理中的关联。
4. **"Optimization of SSNA1 recombinant protein production in E. coli for biochemical assays"**
- Author: Kumar, S. et al.
- 摘要：优化大肠杆菌表达系统以提高SSNA1重组蛋白产率，并验证其用于体外微管聚合/解聚实验的功能活性。
注：以上文献为示例，实际引用需根据具体研究内容检索PubMed或Web of Science等数据库。
SSNA1 (Spindle and Centrosome Associated Protein 1), also known as NA14 or DC13. is a microtubule-associated protein implicated in regulating cellular division and cytoskeletal dynamics. It was initially identified through its association with the mitotic spindle and centrosomes during cell cycle progression. The protein is evolutionarily conserved and expressed ubiquitously in human tissues, with higher levels observed in proliferating cells. Structurally, SSNA1 contains a central coiled-coil domain critical for self-oligomerization and interaction with microtubules, enabling its role in stabilizing microtubule networks and facilitating spindle assembly during mitosis.
Research highlights SSNA1's involvement in multiple cellular processes, including ciliogenesis, cell migration, and intracellular trafficking. Its dysfunction has been linked to pathological conditions such as cancer and autoimmune diseases. For instance, SSNA1 overexpression promotes tumor cell invasion and metastasis in certain cancers by enhancing microtubule stability and actin remodeling. In systemic lupus erythematosus (SLE), SSNA1 is a target of autoantibodies, suggesting its potential role in autoimmune pathogenesis.
Recombinant SSNA1 protein is typically produced using bacterial or mammalian expression systems, enabling in vitro studies of its biochemical properties, interaction partners, and functional mechanisms. Purified recombinant SSNA1 serves as a tool for investigating microtubule dynamics, screening therapeutic agents, or generating antibodies for diagnostic applications. Recent studies also explore its utility in deciphering cilia-related disorders and developing targeted therapies. As a multifunctional regulator of cytoskeletal architecture, SSNA1 remains a focus of both basic research and translational medicine.
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