| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STX10 |
| Uniprot No | O60499 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-228aa |
| 氨基酸序列 | SLEDPFFVVRGEVQKAVNTARGLYQRWCELLQESAAVGREELDWTTNELRNGLRSIEWDLEDLEETIGIVEANPGKFKLPAGDLQERKVFVERMREAVQEMKDHMVSPTAVAFLERNNREILAGKPAAQKSPSDLLDASAVSATSRYIEEQQATQQLIMDEQDQQLEMVSGSIQVLKHMSGRVGEELDEQGIMLDAFAQEMDHTQSRMDGVLRKLAKVSHMTSDRRQ |
| 预测分子量 | 41.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STX10(Syntaxin 10)重组蛋白的3篇代表性文献的简要信息：
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1. **文献名称**：*Syntaxin 10 regulates endosome-to-Golgi transport by interacting with Vps45*
**作者**：Wang H, et al.
**摘要**：研究揭示了STX10通过结合Vps45蛋白调控内体到高尔基体的逆向运输过程，利用重组STX10蛋白证明其与SNARE复合体的相互作用对细胞内脂质运输的关键作用。
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2. **文献名称**：*Structural characterization of human Syntaxin 10 and its role in insulin-stimulated glucose uptake*
**作者**：Zhang Y, et al.
**摘要**：通过重组表达人源STX10蛋白，解析其结构域功能，发现STX10在胰岛素信号通路中调控GLUT4囊泡的膜融合，影响脂肪细胞葡萄糖摄取。
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3. **文献名称**：*Dysregulation of Syntaxin 10 in lysosomal storage disorders*
**作者**：Gupta A, et al.
**摘要**：利用重组STX10蛋白研究溶酶体贮积症中的运输缺陷，发现STX10表达异常导致溶酶体酶分选障碍，提示其病理学意义。
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注：以上文献信息为模拟示例，实际文献需通过PubMed或Web of Science以关键词“Syntaxin 10 recombinant”检索确认。
The STX10 recombinant protein is derived from the human STX10 gene, which encodes syntaxin-10. a member of the syntaxin family of membrane-trafficking proteins. Syntaxins are critical components of the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex, facilitating intracellular vesicle fusion and cargo transport between organelles. STX10 specifically localizes to the endosomal-trans-Golgi network (TGN) interface, playing a role in retrograde transport, lipid homeostasis, and lysosomal function. It interacts with other SNARE proteins, such as Vti1a and VAMP3. to mediate membrane docking and fusion events.
Recombinant STX10 is engineered using expression systems (e.g., E. coli or mammalian cells) to produce purified, biologically active protein for research. Its production typically involves cloning the STX10 cDNA into expression vectors, followed by affinity chromatography (e.g., His-tag purification) and quality validation via SDS-PAGE or Western blot. Researchers utilize this protein to study membrane trafficking mechanisms, organelle dynamics, and diseases linked to syntaxin dysregulation, such as cancer, neurodegenerative disorders, and lysosomal storage diseases. For instance, STX10 overexpression has been implicated in melanoma progression, while its dysfunction may disrupt cholesterol distribution in cells.
The protein’s role in regulating endosome-TGN communication also makes it relevant for investigating viral entry pathways and drug delivery systems. Its recombinant form enables in vitro binding assays, structural studies, and antibody development, providing tools to dissect its molecular interactions and therapeutic potential. Despite its niche focus, STX10 exemplifies how syntaxins bridge cellular logistics with disease pathology, driving interest in both basic and applied biomedical research.
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