| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSPAN2 |
| Uniprot No | O60636 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 112-188aa |
| 氨基酸序列 | GKGVAIRHVQTMYEEAYNDYLKDRGKGNGTLITFHSTFQCCGKESSEQVQPTCPKELLGHKNCIDEIETIISVKLQL |
| 预测分子量 | 8.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟的参考文献示例(非真实文献，建议通过学术数据库查询真实研究)：
1. **"TSPAN2重组蛋白在胶质母细胞瘤侵袭中的作用" - Smith et al., 2018**
摘要：研究通过表达纯化TSPAN2重组蛋白，发现其通过调控整合素信号通路抑制胶质母细胞瘤细胞的迁移和侵袭能力。
2. **"TSPAN2重组蛋白与神经元轴突再生的关联" - Zhang et al., 2020**
摘要：利用原核系统表达TSPAN2重组蛋白，证明其可促进损伤后神经元轴突再生，可能通过激活PI3K/AKT通路实现。
3. **"TSPAN2重组蛋白的晶体结构解析及功能域分析" - Jones et al., 2019**
摘要：首次报道TSPAN2重组蛋白的晶体结构，揭示其跨膜区域与配体结合的关键氨基酸位点，为靶向药物设计提供依据。
4. **"TSPAN2重组蛋白在乳腺癌转移中的调控机制" - Wang et al., 2021**
摘要：通过体外实验发现，TSPAN2重组蛋白可通过结合金属蛋白酶(MMPs)抑制乳腺癌细胞的转移，提示其潜在治疗价值。
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建议通过 **PubMed** 或 **Google Scholar** 搜索真实文献，关键词：**TSPAN2 recombinant protein**, **TSPAN2 function**, **tetraspanin family**.
TSPAN2. a member of the tetraspanin family, is a transmembrane protein characterized by four conserved hydrophobic domains that anchor it to the cell membrane. These proteins play roles in cell adhesion, motility, and signal transduction by organizing membrane complexes. The TSPAN2 gene encodes a 25-30 kDa glycoprotein expressed in various tissues, including the nervous system, where it has been linked to neural development and myelination. Its recombinant form is engineered for in vitro studies, typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) or bacterial systems with codon optimization to ensure proper folding and post-translational modifications.
Research suggests TSPAN2 interacts with integrins and other tetraspanins to regulate cellular processes such as axon guidance, synapse formation, and tumor progression. In cancer, TSPAN2 overexpression correlates with metastasis in certain malignancies, potentially through modulating extracellular matrix interactions. Neurologically, it may influence oligodendrocyte differentiation, impacting diseases like multiple sclerosis. Recombinant TSPAN2 proteins enable functional studies—antibody development, protein-protein interaction assays, and mechanistic exploration of its role in membrane dynamics. Purification often involves affinity tags (e.g., His-tag) followed by chromatography, with quality checks via SDS-PAGE and Western blot. Challenges include maintaining solubility due to its hydrophobic domains, often addressed with detergent buffers. Current studies focus on its therapeutic potential as a biomarker or drug target.
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