| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RUVBL2 |
| Uniprot No | Q9Y230 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-463aa |
| 氨基酸序列 | ATVTATTKVPEIRDVTRIERIGAHSHIRGLGLDDALEPRQASQGMVGQLAARRAAGVVLEMIREGKIAGRAVLIAGQPGTGKTAIAMGMAQALGPDTPFTAIAGSEIFSLEMSKTEALTQAFRRSIGVRIKEETEIIEGEVVEIQIDRPATGTGSKVGKLTLKTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSPHGIPIDLLDRLLIVSTTPYSEKDTKQILRIRCEEEDVEMSEDAYTVLTRIGLETSLRYAIQLITAASLVCRKRKGTEVQVDDIKRVYSLFLDESRSTQYMKEYQDAFLFNELKGETMDTS |
| 预测分子量 | 67.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RUVBL2重组蛋白的3-4条参考文献示例(文献名称与内容为虚构，仅供示例参考)：
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1. **文献名称**: *Structural and Functional Analysis of Recombinant RUVBL2 in ATP-Dependent Chromatin Remodeling*
**作者**: Miller, J. et al.
**摘要**: 本研究通过重组表达人源RUVBL2蛋白，结合冷冻电镜技术解析其与RUVBL1形成的异源二聚体结构，揭示了其ATP水解活性在染色质重塑复合体(如INO80)中的调控机制。
2. **文献名称**: *RUVBL2 Recombinant Protein Enhances DNA Repair Efficiency in Cancer Cell Models*
**作者**: Chen, L. et al.
**摘要**: 利用重组RUVBL2蛋白进行体外功能实验，发现其通过调控ATM/ATR信号通路促进DNA损伤修复，为癌症治疗中靶向RUVBL2的策略提供理论依据。
3. **文献名称**: *Biochemical Characterization of Recombinant RUVBL2's Role in Telomerase Assembly*
**作者**: Garcia, S. et al.
**摘要**: 通过纯化重组RUVBL2蛋白，证明其作为分子伴侣参与端粒酶催化核心(TERT)的组装过程，并阐明了其AAA+结构域在此过程中的关键作用。
4. **文献名称**: *RUVBL2 Recombinant Protein as a Biomarker for Metastatic Tumors*
**作者**: Wang, Y. et al.
**摘要**: 基于重组RUVBL2蛋白的免疫学检测显示，其在多种转移性肿瘤组织中显著高表达，提示其作为预后标志物的潜在临床应用价值。
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*注：以上文献为模拟内容，实际研究需通过学术数据库(如PubMed、Web of Science)检索。建议使用关键词“RUVBL2 recombinant protein”、“RUVBL2 chromatin remodeling”或“RUVBL2 structure”查找真实文献。*
RUVBL2 (RuvB-like ATPase 2), also known as TIP48 or Reptin, is a conserved AAA+ (ATPases Associated with diverse cellular Activities) family protein that plays critical roles in chromatin remodeling, transcriptional regulation, and DNA damage repair. It forms a heterohexameric complex with its paralog RUVBL1 (TIP49/Pontin), functioning as an ATP-dependent molecular chaperone to regulate the assembly and activity of multi-protein complexes like INO80. SWR1. and TIP60 histone acetyltransferase complexes. These complexes are essential for nucleosome repositioning, histone variant exchange, and transcriptional co-activation.
RUVBL2 is involved in diverse cellular processes, including cell cycle progression, ribosome biogenesis, and telomerase maintenance. Dysregulation of RUVBL2 has been linked to cancer progression, as it promotes oncogenic signaling pathways (e.g., c-Myc, β-catenin) and enhances cell proliferation. Its overexpression is observed in various malignancies, making it a potential therapeutic target.
Recombinant RUVBL2 proteins are typically produced in bacterial (e.g., E. coli) or mammalian expression systems for structural and functional studies. The protein contains conserved Walker A/B ATPase motifs and forms ring-shaped oligomers. Purification often involves affinity tags (e.g., His-tag) followed by size-exclusion chromatography. Recombinant variants enable studies on ATPase activity, protein-protein interactions, and inhibitor screening. Current research focuses on resolving its 3D structure, dissecting its role in chromatin remodeling complexes, and developing small-molecule inhibitors for cancer therapy. Challenges include understanding context-dependent regulatory mechanisms and minimizing off-target effects during therapeutic intervention.
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