| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL15 |
| Uniprot No | P61313 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-204aa |
| 氨基酸序列 | GAYKYIQELWRKKQSDVMRFLLRVRCWQYRQLSALHRAPRPTRPDKARRLGYKAKQGYVIYRIRVRRGGRKRPVPKGATYGKPVHHGVNQLKFARSLQSVAEERAGRHCGALRVLNSYWVGEDSTYKFFEVILIDPFHKAIRRNPDTQWITKPVHKHREMRGLTSAGRKSRGLGKGHKFHHTIGGSRRAAWRRRNTLQLHRYR |
| 预测分子量 | 51.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPL15重组蛋白的模拟参考文献示例(仅供参考，建议通过学术数据库核实具体文献)：
---
1. **标题**: *"Recombinant RPL15 Expression in E. coli and Its Role in Ribosome Biogenesis"*
**作者**: Müller, J. et al.
**摘要**: 研究报道了RPL15重组蛋白在大肠杆菌中的高效表达和纯化方法，并证明其在体外核糖体组装过程中对60S亚基的稳定性起关键作用。
2. **标题**: *"RPL15 Regulates c-Myc Translation in Colorectal Cancer via Recombinant Protein Interaction Studies"*
**作者**: Zhang, Y. et al.
**摘要**: 通过重组RPL15蛋白的功能实验，发现其与eIF4E结合，促进原癌基因c-Myc的翻译，从而驱动结直肠癌细胞增殖。
3. **标题**: *"Structural Analysis of Human RPL15 Reveals a Unique RNA-Binding Domain"*
**作者**: Lee, S. et al.
**摘要**: 利用重组RPL15的晶体结构解析，揭示了其C端结构域与28S rRNA特异性结合的分子机制，为核糖体功能研究提供结构基础。
4. **标题**: *"RPL15 Recombinant Protein as a Potential Biomarker for Multiple Myeloma Progression"*
**作者**: Rossi, M. et al.
**摘要**: 研究发现RPL15在多发性骨髓瘤中过表达，重组蛋白的体外实验表明其通过激活NF-κB通路促进肿瘤细胞存活。
---
**注意**：以上文献为模拟示例，实际研究请通过PubMed、Web of Science或Google Scholar等平台检索关键词“RPL15 recombinant protein”或“RPL15 functional studies”获取。
**Background of Recombinant RPL15 Protein**
Recombinant RPL15 protein is a engineered version of the human ribosomal protein L15 (RPL15), a critical component of the 60S ribosomal subunit involved in protein synthesis. RPL15 plays a structural and functional role in ribosome assembly and mRNA translation, ensuring proper cellular protein production. Dysregulation of RPL15 has been linked to various diseases, including cancers such as colorectal carcinoma, leukemia, and breast cancer, where its overexpression or mutation may drive tumor progression by altering ribosome biogenesis or promoting oncogenic signaling pathways.
The recombinant form of RPL15 is typically produced using heterologous expression systems like *E. coli* or mammalian cell lines. This allows large-scale production of the protein with high purity, often tagged with affinity markers (e.g., His-tag) for simplified purification via chromatography. Researchers utilize recombinant RPL15 to study its molecular interactions, structural contributions to ribosome integrity, and functional roles in translation regulation. It also serves as a tool for investigating disease mechanisms, screening therapeutic agents, or developing diagnostic biomarkers.
Additionally, recombinant RPL15 is valuable in structural biology (e.g., cryo-EM studies) to visualize ribosome architecture and in functional assays to dissect its role in cellular stress responses or apoptosis. Its study contributes to understanding ribosomopathies and cancer biology, highlighting its potential as a therapeutic target.
×
