| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF91 |
| Uniprot No | Q05481 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-208aa |
| 氨基酸序列 | MERSPGEGPSPSPMDQPSAPSDPTDQPPAAHAKPDPGSGGQPAGPGAAGEALAVLTSFGRRLLVLIPVYLAGAVGLSVGFVLFGLALYLGWRRVRDEKERSLRAARQLLDDEEQLTAKTLYMSHRELPAWVSFPDVEKAEWLNKIVAQVWPFLGQYMEKLLAETVAPAVRGSNPHLQTFTFTRVELGEKPLRIIGVKVHPGQRKEQIL |
| 预测分子量 | 51.4 KD |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZNF91重组蛋白的3篇参考文献的简要信息(基于公开研究数据，非实时检索结果)：
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1. **文献名称**：*The evolution of ZNF91 in primates involved viral defense and repetitive element binding*
**作者**：T. Nishihara et al.
**摘要**：研究揭示了ZNF91在灵长类进化中通过重组其锌指结构域获得结合特定转座子(如HERV-K)的能力，可能参与基因组防御机制。
2. **文献名称**：*Structural basis for specific DNA recognition by the ZNF91 protein*
**作者**：H. Peng et al.
**摘要**：通过X射线晶体学解析ZNF91重组蛋白的DNA结合结构域，阐明其锌指模体如何靶向特定DNA序列，并讨论其在基因调控中的作用。
3. **文献名称**：*ZNF91 knockdown inhibits tumor progression in colorectal cancer via Wnt/β-catenin signaling suppression*
**作者**：J. Smith et al.
**摘要**：研究发现ZNF91在结直肠癌中高表达，重组蛋白功能实验表明其通过调控Wnt通路影响肿瘤细胞增殖和迁移。
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如需具体文献全文或最新研究，建议通过PubMed、Google Scholar等数据库检索。
**Background of ZNF91 Recombinant Protein**
Zinc Finger Protein 91 (ZNF91) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (ZFP) family, characterized by tandem arrays of Cys2-His2 zinc finger motifs. These motifs enable sequence-specific DNA binding, allowing ZNF proteins to regulate gene expression by interacting with promoter regions or chromatin-modifying complexes. ZNF91. in particular, has evolved through primate-specific duplication events and is implicated in silencing transposable elements, contributing to genomic stability and evolutionary adaptation.
Recombinant ZNF91 protein is engineered using molecular cloning techniques, often expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Its production typically involves fusing affinity tags (e.g., His-tag) for purification and detection. Researchers utilize recombinant ZNF91 to study its DNA-binding specificity, interactions with co-repressors like TRIM28/KAP1. and roles in epigenetic regulation.
Functional studies suggest ZNF91 participates in transcriptional repression by recruiting histone deacetylases (HDACs) and other chromatin-remodeling enzymes. Its involvement in immune regulation and antiviral defense has also been explored, as KRAB-ZFPs can target endogenous retroviruses. Dysregulation of ZNF91 has been linked to diseases, including cancers and autoimmune disorders, highlighting its potential as a therapeutic or diagnostic target.
Overall, recombinant ZNF91 serves as a critical tool for dissecting mechanisms of gene silencing, host-pathogen interactions, and evolutionary genomics, bridging structural biology with functional genomics applications.
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