| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS11 |
| Uniprot No | P62280 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-158aa |
| 氨基酸序列 | ADIQTERAYQKQPTIFQNKKRVLLGETGKEKLPRYYKNIGLGFKTPKEAIEGTYIDKKCPFTGNVSIRGRILSGVVTKMKMQRTIVIRRDYLHYIRKYNRFEKRHKNMSVHLSPCFRDVQIGDIVTVGECRPLSKTVRFNVLKVTKAAGTKKQFQKF |
| 预测分子量 | 45.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS11重组蛋白的3篇文献摘要示例(注：文献为虚构示例，实际文献需通过学术数据库检索)：
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1. **文献名称**：*Expression and Purification of Recombinant RPS11 in Escherichia coli for Structural Studies*
**作者**：Zhang Y, et al.
**摘要**：本研究报道了通过大肠杆菌表达系统高效制备重组RPS11蛋白的方法，优化了诱导条件和纯化步骤(镍柱亲和层析)，获得了高纯度蛋白用于后续X射线晶体学分析，为解析RPS11的分子结构奠定基础。
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2. **文献名称**：*RPS11 Overexpression Promotes Tumor Progression via Ribosome Biogenesis in Colorectal Cancer*
**作者**：Wang L, et al.
**摘要**：通过重组RPS11蛋白体外功能实验，发现其在结直肠癌细胞中异常高表达可激活核糖体合成通路，促进细胞增殖和迁移，提示RPS11可能作为癌症治疗潜在靶点。
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3. **文献名称**：*Functional Analysis of RPS11 Mutations Linked to Diamond-Blackfan Anemia*
**作者**：Kumar S, et al.
**摘要**：研究利用重组突变型RPS11蛋白，揭示了其在核糖体组装中的功能缺陷，阐明了RPS11基因突变导致先天性红细胞再生障碍性贫血(DBA)的分子机制，为疾病治疗提供新思路。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词：
`"RPS11" AND ("recombinant protein" OR "expression" OR "ribosome")`，筛选近5年高被引研究。
RPS11 (Ribosomal Protein S11) is a component of the 40S small ribosomal subunit, playing a critical role in mRNA decoding during protein synthesis. As part of the evolutionarily conserved S17P family, it contributes to ribosome assembly and stability. Beyond its canonical role in translation, RPS11 has been implicated in non-ribosomal functions, including cell proliferation, apoptosis, and DNA repair, highlighting its regulatory versatility.
Recombinant RPS11 protein is engineered using expression systems like *E. coli* or mammalian cells, enabling controlled production for functional studies. Its recombinant form typically retains post-translational modifications when expressed in eukaryotic systems, enhancing biological relevance. Structural studies reveal conserved domains critical for rRNA binding and ribosome integrity, with mutations linked to ribosomopathies such as Diamond-Blackfan anemia (DBA), where impaired ribosome biogenesis disrupts erythroid development.
Emerging research connects RPS11 dysregulation to cancer progression, with overexpression observed in malignancies like hepatocellular carcinoma and leukemia. This has spurred interest in its potential as a therapeutic target or biomarker. Recombinant RPS11 facilitates *in vitro* assays to map protein interactions, dissect ribosome assembly pathways, or screen small molecules targeting ribosome-related pathologies. Furthermore, its role in modulating p53-dependent stress responses underscores its importance in maintaining genomic stability, bridging ribosomal biology to broader cellular homeostasis mechanisms. Ongoing studies aim to unravel its dual roles in health and disease, positioning recombinant RPS11 as a vital tool for both basic and translational research.
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