| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SYNCRIP |
| Uniprot No | O60506 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-191aa |
| 氨基酸序列 | ATEHVNGNGTEEPMDTTSAVIHSENFQTLLDAGLPQKVAEKLDEIYVAGLVAHSDLDERAIEALKEFNEDGALAVLQQFKDSDLSHVQNKSAFLCGVMKTYRQREKQGTKVADSSKGPDEAKIKALLERTGYTLDVTTGQRKYGGPPPDSVYSGQQPSVGTEIFVGKIPRDLFEDELVPLFEKAGPIWDL |
| 预测分子量 | 47.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SYNCRIP重组蛋白的3篇参考文献摘要：
1. **"SYNCRIP regulates the alternative splicing of caspase 9 in hepatocellular carcinoma"**
- **作者**: Liu et al.
- **摘要**: 研究揭示了SYNCRIP通过调控caspase 9的选择性剪接，影响肝癌细胞凋亡和化疗耐药性，重组蛋白实验证实其与RNA结合功能的关键作用。
2. **"SYNCRIP modulates the replication of hepatitis C virus through interactions with viral RNA"**
- **作者**: Wang et al.
- **摘要**: 发现SYNCRIP重组蛋白直接结合丙肝病毒RNA的5'非编码区，通过促进病毒RNA的稳定性增强病毒复制，提示其作为宿主因子在病毒感染中的作用。
3. **"Structural insights into the RNA chaperone activity of SYNCRIP in neuronal mRNA transport"**
- **作者**: Smith et al.
- **摘要**: 利用重组SYNCRIP蛋白解析其RNA伴侣蛋白结构，阐明其通过促进mRNA-蛋白质复合物组装参与神经元内RNA运输的分子机制。
如需具体文献来源或更多信息可进一步查询PubMed或相关数据库。
SYNCRIP (synaptotagmin-binding cytoplasmic RNA-interacting protein), also known as hnRNP Q or NSAP1. is a multifunctional RNA-binding protein belonging to the heterogeneous nuclear ribonucleoprotein (hnRNP) family. It contains three N-terminal RNA recognition motifs (RRMs) and a C-terminal arginine/glycine-rich (RGG) domain, enabling interactions with diverse RNA targets and proteins. Initially identified as a synaptotagmin-binding partner involved in neuronal RNA transport, SYNCRIP has since been recognized as a key regulator of RNA metabolism, including splicing, trafficking, stability, and translation.
Functionally, SYNCRIP participates in viral replication (e.g., HCV, SARS-CoV-2), cellular processes like polarization, and neurodevelopment. It exhibits dual roles in mRNA regulation, either stabilizing transcripts (e.g., NANOG in stem cells) or promoting degradation through miRNA recruitment. In disease contexts, SYNCRIP dysregulation associates with neurological disorders (Alzheimer’s, spinal muscular atrophy) and cancers (leukemia, hepatocellular carcinoma), where it often influences proliferation, metastasis, or therapy resistance.
Recombinant SYNCRIP protein is engineered using bacterial or eukaryotic expression systems for biochemical and functional studies. Its purified form enables exploration of RNA-protein interactions, structural analysis, and screening for therapeutic modulators. Researchers utilize recombinant SYNCRIP to dissect its role in viral life cycles, neuronal RNA granule dynamics, and cancer pathways, highlighting its potential as a diagnostic biomarker or therapeutic target. The protein’s conserved domains and versatile RNA-binding capacity make it a valuable tool for studying post-transcriptional gene regulation mechanisms across biological systems.
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