| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HNRNPD |
| Uniprot No | Q14103 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-355aa |
| 氨基酸序列 | SEEQFGGDGAAAAATAAVGGSAGEQEGAMVAATQGAAAAAGSGAGTGGGTASGGTEGGSAESEGAKIDASKNEEDEGHSNSSPRHSEAATAQREEWKMFIGGLSWDTTKKDLKDYFSKFGEVVDCTLKLDPITGRSRGFGFVLFKESESVDKVMDQKEHKLNGKVIDPKRAKAMKTKEPVKKIFVGGLSPDTPEEKIREYFGGFGEVESIELPMDNKTNKRRGFCFITFKEEEPVKKIMEKKYHNVGLSKCEIKVAMSKEQYQQQQQWGSRGGFAGRARGRGGGPSQNWNQGYSNYWNQGYGNYGYNSQGYGGYGGYDYTGYNNYYGYGDYSNQQSGYGKVSRRGGHQNSYKPY |
| 预测分子量 | 65.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HNRNPD重组蛋白的示例参考文献(注：文献为假设性示例，仅供参考格式)：
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1. **文献名称**：*Recombinant HNRNPD regulates AU-rich element-mediated mRNA decay in vitro*
**作者**：Smith, J., et al.
**摘要**：本研究通过在大肠杆菌中表达重组HNRNPD蛋白，验证其与含AU富集元件的RNA结合能力，并证明其通过招募核酸酶复合体加速靶mRNA的降解。
2. **文献名称**：*Structural characterization of HNRNPD RNA-binding domains using recombinant protein expression*
**作者**：Li, X., & Wang, Y.
**摘要**：利用重组HNRNPD蛋白的晶体结构分析，揭示了其两个RNA识别基序(RRMs)的构象变化，阐明其选择性结合不同RNA底物的分子机制。
3. **文献名称**：*Recombinant HNRNPD modulates alternative splicing in cellular senescence models*
**作者**：Garcia, R., et al.
**摘要**：通过体外递送重组HNRNPD蛋白至衰老细胞，发现其通过调控前体mRNA的剪接模式，影响细胞周期相关基因(如p21)的表达，延缓衰老进程。
4. **文献名称**：*High-yield production of bioactive HNRNPD in insect cells for functional studies*
**作者**：Chen, H., et al.
**摘要**：优化了昆虫细胞表达系统中重组HNRNPD的纯化方法，获得高活性蛋白，并验证其在体外促进肿瘤细胞mRNA稳定性及迁移能力的功能。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词**HNRNPD recombinant protein**或**AUF1 recombinant protein**(HNRNPD别名)。经典研究多涉及其在mRNA稳定性、剪接调控及疾病关联(如癌症、神经退行性疾病)中的作用。
**Background of HNRNPD Recombinant Protein**
Heterogeneous nuclear ribonucleoprotein D (HNRNPD), also known as AU-rich element RNA-binding protein 1 (AUF1), is a member of the hnRNP family, which plays critical roles in RNA metabolism. This multifunctional protein contains RNA recognition motifs (RRMs) that enable binding to AU-rich elements (AREs) commonly found in the 3'-untranslated regions (3'UTRs) of mRNAs, particularly those encoding cytokines, proto-oncogenes, and cell cycle regulators. By interacting with these sequences, HNRNPD regulates mRNA stability, splicing, translation, and degradation, thereby influencing gene expression under various cellular conditions, including stress, inflammation, and differentiation.
HNRNPD is implicated in diverse physiological and pathological processes. It acts as a stress-responsive protein, modulating immune responses and cellular proliferation. Dysregulation of HNRNPD has been linked to cancers, neurodegenerative diseases, and autoimmune disorders. For instance, in cancer, HNRNPD can either promote or suppress tumorigenesis depending on context—stabilizing oncogenic mRNAs or enhancing the decay of tumor suppressor transcripts. Its dual roles highlight its complexity in disease mechanisms.
Recombinant HNRNPD protein is produced using expression systems like *E. coli* or mammalian cells, followed by purification to ensure high specificity and activity. This engineered protein serves as a vital tool for studying RNA-protein interactions, post-transcriptional regulatory mechanisms, and signal transduction pathways *in vitro*. Researchers employ it in techniques such as electrophoretic mobility shift assays (EMSAs), RNA immunoprecipitation (RIP), and structural studies to decipher its molecular functions. Additionally, it aids in drug discovery efforts targeting RNA-binding proteins for therapeutic interventions.
Overall, HNRNPD recombinant protein bridges foundational research and translational applications, offering insights into gene regulation and potential avenues for treating RNA-related diseases.
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