| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS13 |
| Uniprot No | P62277 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-151aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMGRMHAP GKGLSQSALP YRRSVPTWLK LTSDDVKEQI YKLAKKGLTP SQIGVILRDS HGVAQVRFVT GNKILRILKS KGLAPDLPED LYHLIKKAVA VRKHLERNRK DKDAKFRLIL IESRIHRLAR YYKTKRVLPP NWKYESSTAS ALVA |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS13重组蛋白的模拟参考文献示例(仅供参考，建议通过PubMed、Google Scholar等平台查阅真实文献)：
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1. **文献名称**：*"Cloning and Expression of Recombinant RPS13 in Escherichia coli for Structural Studies"*
**作者**：Chen L, et al.
**摘要**：该研究报道了通过基因克隆技术将人源RPS13基因在大肠杆菌中成功表达，并优化了纯化条件。重组蛋白的结晶分析表明其结构与核糖体小亚基的结合位点相关。
2. **文献名称**：*"RPS13 Regulates Oxidative Stress-Induced Apoptosis in Colorectal Cancer Cells"*
**作者**：Wang Y, et al.
**摘要**：研究发现RPS13重组蛋白在结直肠癌细胞中过表达可抑制氧化应激诱导的凋亡，提示其可能通过调控核糖体功能影响肿瘤细胞生存。
3. **文献名称**：*"Structural Insights into RPS13's Role in Ribosome Assembly"*
**作者**：Smith JA, et al.
**摘要**：通过冷冻电镜和突变分析，揭示了RPS13在核糖体小亚基组装中的关键作用，其重组蛋白的特定结构域对18S rRNA的折叠至关重要。
4. **文献名称**：*"A Mutation in RPS13 Causes Diamond-Blackfan Anemia-like Phenotypes in Zebrafish"*
**作者**：Kim H, et al.
**摘要**：利用斑马鱼模型发现RPS13基因突变导致红细胞生成障碍，重组蛋白的功能缺失模拟了类似人类Diamond-Blackfan贫血的表型。
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**注意**：以上为模拟示例，实际文献需通过学术数据库检索。建议结合关键词“RPS13”、“recombinant protein”、“ribosomal protein S13”进一步查找。
**Background of Recombinant RPS13 Protein**
Ribosomal Protein S13 (RPS13) is a core component of the 40S small subunit of eukaryotic ribosomes, playing a critical role in mRNA decoding during protein synthesis. As part of the ribosomal machinery, RPS13 contributes to ribosome assembly, stability, and translational fidelity. Its conserved structure across species underscores its fundamental role in cellular homeostasis. Dysregulation of RPS13 has been linked to various pathologies, including cancer and ribosomopathies, highlighting its importance beyond basic translation.
Recombinant RPS13 protein is engineered using genetic cloning techniques, where the *RPS13* gene is inserted into expression vectors (e.g., *E. coli* or mammalian systems) to produce high-purity, functional protein. This allows researchers to study its structure, interactions, and mechanistic roles in vitro. Recombinant forms are often tagged for ease of purification (e.g., His-tag) and visualization.
Studies on recombinant RPS13 have advanced understanding of ribosome biogenesis defects and their implications in diseases. For example, aberrant RPS13 expression is observed in certain cancers, suggesting potential as a diagnostic marker or therapeutic target. Additionally, mutations in RPS13 are associated with Diamond-Blackfan anemia, a rare genetic disorder characterized by ribosomal dysfunction.
The protein’s recombinant version also facilitates drug discovery, enabling high-throughput screening for compounds modulating ribosome activity or targeting RPS13-related pathways. Furthermore, structural analyses (e.g., X-ray crystallography or cryo-EM) using recombinant RPS13 provide insights into ribosome assembly and function. Overall, recombinant RPS13 serves as a vital tool for exploring both basic ribosomal biology and its translational applications in biomedicine.
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