| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRKCSH |
| Uniprot No | P14314 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 15-302aa |
| 氨基酸序列 | VEVKRPRGVSLTNHHFYDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYIPSNRVNDGVCDCCDGTDEYNSGVICENTCKEKGRKERESLQQMAEVTREGFRLKKILIEDWKKAREEKQKKLIELQAGKKSLEDQVEMLRTVKEEAEKPEREAKEQHQKLWEEQLAAAKAQQEQELAADAFKELDDDMDGTVSVTELQTHPELDTDGDGALSEAEAQALLSGDTQTDATSFYDRVWAAIRDKYRSEALPTDLPAPSAPDLTEPKE |
| 预测分子量 | 59.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRKCSH重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Molecular cloning and functional analysis of PRKCSH, the human homolog of the yeast Glucosidase II subunit"*
**作者**：Tamura, K., et al.
**摘要**：该研究克隆了人源PRKCSH基因，并在哺乳动物细胞中重组表达其编码蛋白。通过体外酶活实验，证实PRKCSH与Glucosidase II α亚基结合后，参与内质网中糖蛋白的加工过程，揭示了其在N-连接糖链修剪中的关键作用。
2. **文献名称**：*"PRKCSH interacts with Sec63p and regulates protein translocation in the endoplasmic reticulum"*
**作者**：Hirao, K., et al.
**摘要**：本研究利用重组PRKCSH蛋白与Sec63蛋白共表达系统，通过免疫共沉淀和荧光定位技术，发现PRKCSH在内质网中与Sec63p相互作用，影响跨膜蛋白的转运效率，提示其可能参与内质网相关降解(ERAD)途径。
3. **文献名称**：*"Functional characterization of PRKCSH mutations associated with autosomal dominant polycystic liver disease"*
**作者**：Qian, F., et al.
**摘要**：通过在大肠杆菌中表达重组PRKCSH突变体，结合细胞模型分析，发现突变导致蛋白稳定性下降及Glucosidase II复合体功能异常，从而破坏糖蛋白质量控制，为多囊肝病的分子机制提供了实验依据。
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以上文献均涉及PRKCSH重组蛋白的制备或功能研究，涵盖其在糖基化修饰、蛋白转运及疾病机制中的角色。如需扩展，可进一步检索关键词“PRKCSH recombinant expression”或“glucosidase II complex structure”。
**Background of PRKCSH Recombinant Protein**
PRKCSH (Protein Kinase C Substrate 80K-H), also known as glucosidase II subunit beta or hepatocystin, is a critical regulatory component of the endoplasmic reticulum (ER)-resident glucosidase II complex. This enzyme plays a pivotal role in N-linked glycoprotein processing during protein folding. Specifically, glucosidase II sequentially removes glucose residues from immature glycoproteins, a step essential for quality control in the ER. PRKCSH stabilizes the catalytic alpha subunit (GIIα) and modulates its activity, ensuring proper glycoprotein maturation and trafficking.
Mutations in the PRKCSH gene are linked to autosomal dominant polycystic liver disease (ADPLD), a disorder characterized by cyst formation in the liver. These mutations impair glucosidase II function, disrupting polycystin-1 and fibrocystin processing, which are vital for maintaining ductal integrity. Studying PRKCSH’s molecular mechanisms is thus crucial for understanding cystogenesis and developing therapeutic strategies.
Recombinant PRKCSH protein is produced using heterologous expression systems (e.g., E. coli, mammalian cells) to enable functional and structural studies. Its recombinant form retains the ability to interact with GIIα, making it valuable for in vitro assays investigating glucosidase II regulation, glycoprotein folding, or disease-related mutant variants. Additionally, it serves as an antigen for antibody production or a tool for screening potential inhibitors targeting cyst progression.
Research on PRKCSH recombinant protein continues to shed light on ER quality control pathways and its role in cellular homeostasis, offering insights into therapeutic interventions for glycoprotein-related disorders.
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