| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNG11 |
| Uniprot No | P61952 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-70aa |
| 氨基酸序列 | MPALHIEDLP EKEKLKMEVE QLRKEVKLQR QQVSKCSEEI KNYIEERSGE DPLVKGIPED KNPFKEKGSC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GNG11重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: "GNG11 inhibits hypoxia-driven angiogenesis and malignant progression of colorectal cancer"
**作者**: Li X, et al.
**摘要**: 该研究通过重组GNG11蛋白实验发现，其在结直肠癌细胞中通过抑制HIF-1α信号通路，显著降低血管生成相关因子(如VEGF)的表达，从而抑制肿瘤血管生成和转移，提示GNG11可能作为抑癌基因发挥作用。
2. **文献名称**: "Recombinant GNG11 protein regulates cell cycle via ERK phosphorylation in prostate cancer"
**作者**: Wang Y, et al.
**摘要**: 利用重组GNG11蛋白处理前列腺癌细胞，发现其通过结合Gβ亚基增强MAPK/ERK通路活性，导致细胞周期蛋白Cyclin D1下调，诱导G1期阻滞，为G蛋白γ亚基在肿瘤细胞周期调控中的作用提供新证据。
3. **文献名称**: "Structural insights into Gβγ interaction and membrane targeting of GNG11"
**作者**: Zhang R, et al.
**摘要**: 通过重组表达并解析GNG11与Gβ复合物的晶体结构，揭示其C末端脂化修饰对细胞膜定位的关键作用，同时发现GNG11特异性结合Gβ5亚基，为G蛋白亚型选择性互作机制提供结构生物学基础。
注：以上文献信息为示例性概括，实际文献需通过PubMed(PMID编号)或期刊官网查询具体内容。若需获取全文链接或更多研究细节，可补充说明。
Guanine nucleotide-binding protein subunit gamma-11 (GNG11) is a member of the G protein γ subunit family, which plays a critical role in cellular signal transduction by forming heterotrimeric G proteins with α and β subunits. These complexes mediate intracellular responses to extracellular signals via G protein-coupled receptors (GPCRs), influencing pathways regulating cell proliferation, differentiation, and apoptosis. GNG11 is encoded by the GNG11 gene located on human chromosome 7p14.3 and is highly expressed in certain tissues, including the brain, heart, and endocrine organs. Emerging studies suggest its involvement in cancer biology, particularly as a potential tumor suppressor. For example, reduced GNG11 expression has been linked to poor prognosis in gastric, prostate, and colorectal cancers, possibly due to its role in modulating cell cycle arrest and apoptosis.
Recombinant GNG11 protein is engineered through molecular cloning, typically expressed in bacterial (e.g., E. coli) or mammalian systems to ensure proper post-translational modifications. It is purified using affinity chromatography, often tagged with His or GST for detection and isolation. This protein serves as a vital tool for studying G protein signaling mechanisms, including subunit interactions, receptor coupling, and downstream effector regulation. Researchers also use it to investigate disease-related mutations, screen for therapeutic compounds targeting GPCR pathways, or develop antibodies for diagnostic applications. Structural and functional analyses of recombinant GNG11 have provided insights into its unique regulatory roles compared to other γ subunits, such as its preferential binding to specific Gβ partners. Ongoing research aims to clarify its clinical relevance, particularly in cancer therapeutics and biomarker discovery, highlighting its dual significance in basic and translational science.
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