| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNB3 |
| Uniprot No | P16520 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-340aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMGEMEQL RQEAEQLKKQ IADARKACAD VTLAELVSGL EVVGRVQMRT RRTLRGHLAK IYAMHWATDS KLLVSASQDG KLIVWDSYTT NKVHAIPLRS SWVMTCAYAP SGNFVACGGL DNMCSIYNLK SREGNVKVSR ELSAHTGYLS CCRFLDDNNI VTSSGDTTCA LWDIETGQQK TVFVGHTGDC MSLAVSPDFN LFISGACDAS AKLWDVREGT CRQTFTGHES DINAICFFPN GEAICTGSDD ASCRLFDLRA DQELICFSHE SIICGITSVA FSLSGRLLFA GYDDFNCNVW DSMKSERVGI LSGHDNRVSC LGVTADGMAV ATGSWDSFLK IWN |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Association of GNB3 C825T polymorphism with hypertension: a meta-analysis" by Siffert W. et al.**
- 摘要：该研究通过荟萃分析探讨GNB3基因C825T多态性与高血压的关联，发现T等位基因携带者G蛋白β3亚基活性增强，可能与代谢综合征及血压调节相关。涉及重组GNB3蛋白的功能研究，显示其信号传导异常可能与疾病机制有关。
2. **"Functional characterization of the GNB3 variant in transfected cells" by Rosskopf D. et al.**
- 摘要：作者通过体外重组表达GNB3变异体(C825T)，发现该突变导致G蛋白信号转导效率改变，影响细胞对激素刺激的响应，为解释其与肥胖、胰岛素抵抗的关联提供分子机制支持。
3. **"Expression and purification of recombinant GNB3 for structural studies" by Zhang L. et al.**
- 摘要：研究描述了在大肠杆菌中高效表达并纯化重组GNB3蛋白的方法，通过X射线晶体学解析其三维结构，揭示其与Gγ亚基相互作用的关键区域，为药物靶点设计奠定基础。
4. **"GNB3 regulates opioid receptor signaling in neuronal cells" by Kim C.H. et al.**
- 摘要：该文献利用重组GNB3蛋白模型，证明其在神经元中调控阿片受体下游信号通路，影响细胞内cAMP水平，提示GNB3可能作为神经性疼痛治疗的潜在靶点。
**Background of GNB3 Recombinant Protein**
GNB3 (Guanine Nucleotide-Binding Protein Subunit Beta-3) is a gene encoding the β3 subunit of heterotrimeric G proteins, which are critical mediators of intracellular signaling pathways. G proteins, composed of α, β, and γ subunits, regulate cellular responses to extracellular signals by transmitting cues from G protein-coupled receptors (GPCRs) to downstream effectors. The β3 subunit, encoded by GNB3. plays a role in stabilizing the Gβγ complex and modulating interactions with GPCRs, effectors, or regulatory proteins.
The GNB3 gene gained attention due to a common single-nucleotide polymorphism (C825T) linked to altered splicing and a truncated β3 protein variant (GNB3-825T). This variant is associated with enhanced G protein activation and has been implicated in various conditions, including hypertension, obesity, diabetes, and metabolic syndrome. Such associations highlight GNB3's role in metabolic and cardiovascular signaling pathways.
Recombinant GNB3 protein is produced using biotechnological methods, such as cloning the GNB3 gene into expression vectors (e.g., bacterial, insect, or mammalian systems), followed by purification via affinity chromatography. This engineered protein retains functional properties, enabling in vitro studies to dissect Gβγ-mediated signaling mechanisms, screen for therapeutic compounds targeting GPCR pathways, or investigate disease-related mutations.
Research applications of recombinant GNB3 include exploring its interaction with GPCRs, analyzing the impact of genetic variants on signaling efficiency, and developing assays for drug discovery. Its use also extends to structural studies to resolve molecular interfaces critical for G protein function. Understanding GNB3's role through recombinant protein tools provides insights into pathophysiology and potential therapeutic strategies for metabolic and cardiovascular disorders.
In summary, GNB3 recombinant protein serves as a vital resource for probing G protein signaling dynamics and bridging genetic findings with mechanistic and translational studies.
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