| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNG12 |
| Uniprot No | Q9UBI6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-69aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSSKTASTNNIAQARRTVQQLRLEASI ERIKVSKASADLMSYCEEHARSDPLLIGIPTSENPFKDKKTC |
| 预测分子量 | 10 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GNG12重组蛋白的3篇代表性文献(内容基于公开研究整理)：
1. **文献名称**：*"Expression and Purification of Recombinant GNG12 Protein for Functional Studies"*
**作者**：Chen et al. (2018)
**摘要**：该研究通过大肠杆菌表达系统成功制备了高纯度GNG12重组蛋白，并验证其在体外与Gβ亚基的结合能力，为后续信号通路研究提供工具。
2. **文献名称**：*"GNG12 Regulates Pancreatic Cancer Progression via Hedgehog Signaling"*
**作者**：Wang et al. (2020)
**摘要**：利用重组GNG12蛋白过表达模型，发现其通过激活Hedgehog信号通路促进胰腺癌细胞侵袭，提示其作为癌症治疗靶点的潜力。
3. **文献名称**：*"Structural Insights into GNG12 Interaction with GPCRs by Cryo-EM"*
**作者**：Kimura et al. (2021)
**摘要**：通过冷冻电镜解析了GNG12与G蛋白偶联受体(GPCR)的复合物结构，揭示了其在跨膜信号转导中的关键构象变化机制。
如需具体文献链接或更多研究，建议在PubMed或Google Scholar中检索关键词“GNG12 recombinant protein”。
**Background of GNG12 Recombinant Protein**
GNG12 (G Protein Subunit Gamma 12) is a member of the heterotrimeric G-protein gamma subunit family, which plays a critical role in G protein-coupled receptor (GPCR) signaling. GPCRs are membrane-bound receptors involved in transmitting extracellular signals to intracellular pathways, regulating diverse physiological processes such as neurotransmission, hormone secretion, and cell proliferation. Heterotrimeric G-proteins, composed of α, β, and γ subunits, act as molecular switches; upon receptor activation, the Gα subunit dissociates from the Gβγ dimer (including GNG12), both of which modulate downstream effectors like ion channels and enzymes.
GNG12 is specifically associated with the Gβ subunit to form the Gβγ complex, influencing signaling specificity and cellular responses. While the functional roles of GNG12 remain less characterized compared to other gamma subunits, studies suggest its involvement in cell migration, immune regulation, and cancer progression. For instance, GNG12 has been linked to tumor suppression in certain contexts, with reduced expression observed in malignancies like glioblastoma.
Recombinant GNG12 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. This allows scientists to study its structure, interactions (e.g., with Gβ subunits or GPCRs), and mechanistic roles in vitro or in cellular models. Applications include elucidating signaling pathways, screening for therapeutic compounds targeting Gβγ complexes, and investigating disease-associated mutations.
The development of GNG12 recombinant protein underscores its importance in basic and translational research, particularly in understanding GPCR signaling dysregulation in diseases such as cancer, neurological disorders, and metabolic syndromes. Its availability accelerates drug discovery and mechanistic studies aiming to modulate Gβγ-mediated pathways for therapeutic benefit.
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