| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXN1 |
| Uniprot No | O15353 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-648aa |
| 氨基酸序列 | MVSLPPPQSDVTLPGPTRLEGERQGDLMQAPGLPGSPAPQSKHAGFSCSSFVSDGPPERTPSLPPHSPRIASPGPEQVQGHCPAGPGPGPFRLSPSDKYPGFGFEEAAASSPGRFLKGSHAPFHPYKRPFHEDVFPEAETTLALKGHSFKTPGPLEAFEEIPVDVAEAEAFLPGFSAEAWCNGLPYPSQEHGPQVLGSEVKVKPPVLESGAGMFCYQPPLQHMYCSSQPPFHQYSPGGGSYPIPYLGSSHYQYQRMAPQASTDGHQPLFPKPIYSYSILIFMALKNSKTGSLPVSEIYNFMTEHFPYFKTAPDGWKNSVRHNLSLNKCFEKVENKSGSSSRKGCLWALNPAKIDKMQEELQKWKRKDPIAVRKSMAKPEELDSLIGDKREKLGSPLLGCPPPGLSGSGPIRPLAPPAGLSPPLHSLHPAPGPIPGKNPLQDLLMGHTPSCYGQTYLHLSPGLAPPGPPQPLFPQPDGHLELRAQPGTPQDSPLPAHTPPSHSAKLLAEPSPARTMHDTLLPDGDLGTDLDAINPSLTDFDFQGNLWEQLKDDSLALDPLVLVTSSPTSSSMPPPQPPPHCFPPGPCLTETGSGAGDLAAPGSGGSGALGDLHLTTLYSAFMELEPTPPTAPAGPSVYLSPSSKPVALA |
| 预测分子量 | 73.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与FOXN1重组蛋白相关的参考文献(注：文献信息为模拟示例，实际引用请核实原文)：
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1. **文献名称**: *FOXN1 recombinant protein promotes thymic epithelial cell differentiation in vitro*
**作者**: Smith J, et al.
**摘要**: 本研究利用大肠杆菌表达系统成功纯化出功能性FOXN1重组蛋白，并证明其能诱导小鼠胚胎干细胞分化为胸腺上皮祖细胞，为胸腺再生研究提供了体外实验基础。
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2. **文献名称**: *Structural and functional analysis of human FOXN1 transcription factor*
**作者**: Li X, Chen Z.
**摘要**: 通过重组FOXN1蛋白的晶体结构解析，揭示了其DNA结合域的关键氨基酸残基，并验证了该蛋白通过调控AIRE基因表达参与T细胞中枢耐受的分子机制。
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3. **文献名称**: *Recombinant FOXN1 enhances hair follicle regeneration in a murine model*
**作者**: Gupta R, et al.
**摘要**: 局部注射FOXN1重组蛋白显著促进脱毛小鼠模型的毛囊再生，表明其在皮肤组织修复中的潜在应用价值，可能与Wnt/β-catenin信号通路激活相关。
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如需具体文献，建议通过PubMed或Web of Science以“FOXN1 recombinant protein”为关键词检索近年研究。
FOXN1 (Forkhead Box N1) is a critical transcription factor primarily known for its role in thymus development and T-cell differentiation. Encoded by the *FOXN1* gene, this protein is a member of the forkhead box (FOX) family, characterized by a conserved DNA-binding domain. FOXN1 is essential for the differentiation and function of thymic epithelial cells (TECs), which create the microenvironment necessary for T-cell maturation. Mutations in *FOXN1* are linked to severe immunodeficiency disorders, such as nude/SCID syndrome in humans, characterized by hairlessness and a lack of functional T cells.
Recombinant FOXN1 protein is engineered through genetic modification, typically expressed in bacterial (e.g., *E. coli*) or mammalian cell systems. This protein retains the functional domains of native FOXN1. including its DNA-binding forkhead domain, enabling it to regulate target genes involved in thymic epithelial development and immune cell signaling. Researchers utilize recombinant FOXN1 to study its molecular mechanisms, including its interaction with co-factors like Wnt/β-catenin pathways, and its role in maintaining thymic architecture and function.
In applied research, recombinant FOXN1 holds therapeutic potential. It is explored in regenerative medicine to restore thymus function in aging or immunocompromised individuals, as thymic involution contributes to weakened immunity. Additionally, it may aid in *in vitro* T-cell generation for adoptive immunotherapies. Studies also investigate its utility in tissue engineering, such as bioengineered thymus transplants for immunodeficiency disorders. Beyond immunology, FOXN1 is implicated in skin and hair follicle development, linking it to dermatological research. However, challenges remain in optimizing delivery systems and ensuring precise regulatory control *in vivo*. Current work focuses on enhancing protein stability and activity to translate laboratory findings into clinical applications.
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