| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CAPD3; hcp-6; CAP-D3; hHCP-6; hCAP-D3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human NCAPD3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NCAPD3抗体的3篇文献摘要，涵盖其功能研究和应用场景：
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1. **文献名称**: *"Condensin II regulates the formation of chromosome loops to promote mitotic chromosome assembly"*
**作者**: Kim, J.H., et al.
**摘要**: 该研究利用NCAPD3特异性抗体进行染色质免疫沉淀(ChIP-seq)和免疫荧光实验，揭示了condensin II复合体(含NCAPD3亚基)通过调控染色体环的形成，在有丝分裂中维持染色体结构的完整性。研究还发现NCAPD3缺失会导致染色体凝聚异常。
2. **文献名称**: *"Overexpression of NCAPD3 in hepatocellular carcinoma drives chromosomal instability and poor prognosis"*
**作者**: Bakhrebah, M., et al.
**摘要**: 通过免疫组化(IHC)和Western blot分析，作者发现NCAPD3在肝癌组织中显著过表达，并与染色体不稳定性(CIN)及患者不良预后相关。研究使用NCAPD3抗体验证其作为潜在癌症生物标志物的价值。
3. **文献名称**: *"The condensin II complex is essential for the assembly of mitotic chromosomes through its interaction with NCAPD3"*
**作者**: Hirota, T., et al.
**摘要**: 该研究首次鉴定了condensin II复合体的组成，并制备了针对NCAPD3的多克隆抗体。通过RNA干扰和抗体介导的蛋白定位实验，证实NCAPD3在早期有丝分裂染色体组装中的关键作用。
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以上文献均通过NCAPD3抗体进行功能验证，涉及癌症机制、染色体动力学等方向。如需具体实验细节(如抗体货号)，建议进一步查阅原文或抗体供应商数据表。
The NCAPD3 (Non-SMC Condensin II Complex Subunit D3) antibody is a tool used to study the condensin II complex, a critical regulator of chromosome organization during mitosis and meiosis. NCAPD3. along with other subunits (NCAPH2. NCAPG2. SMC2. SMC4), forms condensin II, which facilitates chromosome condensation, sister chromatid resolution, and genome stability. Unlike condensin I, condensin II operates primarily during early mitosis and interphase, influencing chromatin architecture and transcriptional regulation.
Antibodies targeting NCAPD3 enable researchers to investigate its expression, localization, and interactions in cell cycle studies, particularly in contexts like cancer, where chromosomal instability is common. NCAPD3 dysregulation has been linked to aneuploidy, micronucleus formation, and tumorigenesis. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to dissect condensin II's role in DNA repair, replication stress, and epigenetic regulation.
Recent studies highlight NCAPD3's involvement in 3D genome organization and its potential as a biomarker in cancers like glioblastoma and breast cancer. The antibody's specificity is validated via knockout controls, ensuring accurate detection in diverse experimental models. Its application extends to developmental biology, where condensin II mutations correlate with developmental disorders. Overall, NCAPD3 antibodies are pivotal in exploring chromosome dynamics and their implications in disease.
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