| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | ANF; ANP; PND; ATFB6; CDD-ANF |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NPPA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CARD8抗体的3篇代表性文献(虚拟示例，需根据实际文献验证)：
1. **"CARD8 regulates NLRP3 inflammasome activation through caspase-1 interaction"**
- **作者**: Li Y et al.
- **摘要**: 研究揭示了CARD8通过结合caspase-1调控NLRP3炎症小体活化的分子机制，并开发了特异性抗体用于检测其在炎症反应中的表达变化。
2. **"Structural basis of CARD8 autoinhibition and its role in HIV protease sensing"**
- **作者**: Johnson RM et al.
- **摘要**: 利用CARD8抗体进行结构解析，阐明了CARD8的自抑制构象及其在识别HIV蛋白酶介导的细胞焦亡中的关键作用。
3. **"CARD8 expression correlates with cardiovascular disease progression"**
- **作者**: Wang H et al.
- **摘要**: 通过免疫组化分析(使用CARD8特异性抗体)，发现CARD8在动脉粥样硬化患者血管组织中的表达上调，提示其与心血管炎症的潜在关联。
4. **"Antibody-based targeting of CARD8 for inflammatory disease therapy"**
- **作者**: Chen L et al.
- **摘要**: 开发了靶向CARD8的单克隆抗体，并在小鼠模型中验证其通过抑制过度炎症反应缓解类风湿性关节炎的疗效。
**注意**：以上文献标题及内容为示例性质，具体研究需查阅真实数据库(如PubMed、Google Scholar)。建议使用关键词“CARD8 antibody”或“CARD8 inflammasome”检索最新文献。
CARD8 (Caspase activation and recruitment domain 8) is a protein involved in regulating inflammatory responses and apoptosis. It belongs to the CARD protein family, which mediates protein-protein interactions through its conserved CARD domain. Structurally, CARD8 contains an N-terminal pyrin domain (PYD), a central CARD domain, and a C-terminal inhibitory domain that undergoes proteasomal degradation under specific conditions. This degradation releases the N-terminal fragment, enabling its role in inflammasome activation. CARD8 interacts with NLRP3 inflammasome components and modulates caspase-1 activation, influencing the maturation of pro-inflammatory cytokines like IL-1β and IL-18.
CARD8 antibodies are research tools used to study its expression, localization, and molecular interactions in immune signaling pathways. Dysregulation of CARD8 has been implicated in autoimmune and inflammatory diseases, including rheumatoid arthritis, gout, and inflammatory bowel disease. Certain genetic polymorphisms in CARD8 are associated with altered disease susceptibility or severity, highlighting its potential as a biomarker. In therapeutic contexts, targeting CARD8 pathways may offer strategies to modulate aberrant inflammasome activity. However, its dual role in promoting or suppressing inflammation—depending on cellular context and proteolytic processing—complicates its functional characterization. Current studies employ CARD8 antibodies in techniques like Western blotting, immunofluorescence, and co-immunoprecipitation to unravel its mechanisms in health and disease. Further research is needed to clarify its tissue-specific functions and therapeutic potential.
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