| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | CACRC |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CATSPER3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CATSPER3抗体的3篇参考文献(注：以下内容为模拟示例，实际文献需通过学术数据库检索获取)：
1. **文献名称**：*CATSPER3 Antibody Characterization and Its Role in Sperm Motility*
**作者**：Smith A, et al.
**摘要**：本研究通过制备特异性CATSPER3抗体，验证其在人类和小鼠精子中的表达定位，并发现其缺失会导致精子钙离子内流异常及运动能力下降，提示CATSPER3在男性生育中的关键作用。
2. **文献名称**：*Development of a Monoclonal Antibody for Detecting CATSPER3 in Infertility Screening*
**作者**：Chen L, et al.
**摘要**：报道一种新型单克隆抗体的开发，用于检测精液样本中CATSPER3蛋白水平，发现不育症患者中CATSPER3表达显著降低，表明该抗体可作为潜在诊断工具。
3. **文献名称**：*CATSPER3 Channel Complex Assembly Studied via Immunoprecipitation Using Specific Antibodies*
**作者**：Wang Y, et al.
**摘要**：利用CATSPER3抗体进行免疫共沉淀实验，揭示其与CATSPER家族其他亚基(如CATSPER1/2)的相互作用机制，阐明该离子通道复合体的组装模式。
**提示**：实际检索建议使用关键词“CATSPER3 antibody”或“CATSPER3 + sperm + calcium channel”在PubMed/Google Scholar中查找最新研究。部分研究可能侧重通道功能而非抗体开发，需筛选抗体相关应用论文。
The CATSPER3 antibody is a specialized tool used to detect and study the CATSPER3 protein, a key component of the CATSPER (Cation Channel of Sperm) family. CATSPER channels are sperm-specific, pH-sensitive calcium channels essential for sperm hyperactivation, motility, and fertilization. Among the four pore-forming subunits (CATSPER1–4), CATSPER3 forms heteromeric complexes with CATSPER1. 2. and 4. creating a functional calcium channel localized to the sperm principal piece. This channel regulates intracellular calcium influx, driving sperm tail movement and capacitation.
CATSPER3 antibodies are primarily employed in reproductive biology research to investigate male infertility mechanisms, as mutations or dysfunction in CATSPER3 are linked to impaired sperm function and sterility. These antibodies enable protein localization via immunofluorescence, expression analysis through Western blotting, and functional studies in genetically modified models. Their specificity is critical for distinguishing CATSPER3 from other CATSPER subunits and non-target proteins.
Recent studies also explore CATSPER3's potential as a contraceptive target due to its sperm-specific role. Commercial CATSPER3 antibodies are typically raised in rabbits or mice, validated for reactivity in human, mouse, or rat samples. Proper validation using knockout controls ensures reliability in experimental settings, aiding advancements in understanding sperm physiology and therapeutic development.
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