| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | POMC; LPH; MSH; NPP; POC; CLIP |
| Entrez GeneID | 5443 |
| clone | 3A4H4 |
| WB Predicted band size | 29.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ACTH expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于SPN(Sialophorin/CD43)抗体的代表性文献摘要概括，按研究领域分类整理：
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1. **文献名称**：*"Structural characterization of sialophorin (CD43) as a keratin sulfate proteoglycan in human T-lymphoblasts"*
**作者**：Fukuda M. et al.
**摘要**：通过单克隆抗体分析，揭示了SPN(CD43)在T淋巴细胞表面作为硫酸角质素蛋白聚糖的分子结构，证实其N端糖基化修饰与免疫细胞黏附功能相关。
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2. **文献名称**：*"CD43 modulates the differentiation and function of dendritic cells through TLR4 signaling"*
**作者**：Santos-Sierra S. & Corral R.
**摘要**：研究利用抗SPN抗体阻断实验，证明CD43通过TLR4信号通路调控树突状细胞的成熟及抗原呈递能力，影响先天性免疫应答。
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3. **文献名称**：*"SPN/CD43 loss correlates with poor prognosis in diffuse large B-cell lymphoma"*
**作者**：Li Y. et al.
**摘要**：通过免疫组化分析发现，SPN表达缺失与弥漫大B细胞淋巴瘤患者生存率下降显著相关，提示其作为潜在预后标志物的临床价值。
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**领域覆盖**：
- **结构生物学**(文献1解析分子构象)
- **免疫调控**(文献2揭示信号通路机制)
- **肿瘤诊断**(文献3探索临床相关性)
如需特定方向文献补充，可进一步说明研究需求。
**Background of SPN Antibody**
SPN (sialophorin), also known as CD43. is a heavily glycosylated transmembrane protein predominantly expressed on the surface of immune cells, including T cells, B cells, neutrophils, and natural killer (NK) cells. It belongs to the sialomucin family, characterized by extensive O-linked glycosylation and a high content of sialic acid, which confers a strong negative charge. This charge contributes to its role in repelling cell-cell interactions, modulating immune responses, and regulating cell adhesion and migration.
SPN plays a dual role in immune regulation. It acts as both a physical barrier against undesired cellular interactions and a signaling molecule involved in T-cell activation, apoptosis, and cytokine production. Its cytoplasmic domain interacts with cytoskeletal proteins and signaling molecules, influencing intracellular pathways. Dysregulation of SPN expression has been linked to immunodeficiency, autoimmune diseases, and hematologic malignancies, such as leukemia and lymphoma.
SPN antibodies are essential tools in research and diagnostics. They enable the detection of SPN/CD43 in flow cytometry, immunohistochemistry, and Western blotting, aiding in immune cell characterization and disease profiling. In clinical settings, SPN antibodies help diagnose lymphoproliferative disorders, as abnormal SPN expression often serves as a marker for malignant cell populations. Studies continue to explore SPN’s therapeutic potential, including targeting its immunosuppressive effects in cancer.
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