| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAFG |
| Uniprot No | O15525 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-162aa |
| 氨基酸序列 | MTTPNKGNKA LKVKREPGEN GTSLTDEELV TMSVRELNQH LRGLSKEEIV QLKQRRRTLK NRGYAASCRV KRVTQKEELE KQKAELQQEV EKLASENASM KLELDALRSK YEALQTFART VARSPVAPAR GPLAAGLGPL VPGKVAATSV ITIVKSKTDA RS |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
nan
MafG (Musculoaponeurotic Fibrosarcoma Oncogene Homolog G) is a member of the Maf family of transcription factors, which play critical roles in regulating gene expression by binding to antioxidant response elements (ARE) or Maf recognition elements (MARE) in DNA. These proteins are involved in cellular defense mechanisms against oxidative stress, detoxification, and homeostasis. MAFG, specifically, acts as a transcriptional regulator, either activating or repressing target genes depending on its binding partners. For example, it forms heterodimers with nuclear factor erythroid 2-related factor 2 (Nrf2) to activate ARE-driven cytoprotective genes, while partnering with BACH1 or small Maf proteins can repress these pathways. This dual functionality highlights its role in balancing oxidative stress responses and maintaining cellular equilibrium.
Recombinant MAFG protein is engineered for experimental studies to elucidate its molecular interactions, structural properties, and regulatory mechanisms. Produced via expression systems like *E. coli* or mammalian cells, recombinant MAFG retains its DNA-binding capacity and partner-protein interaction domains, enabling in vitro assays such as electrophoretic mobility shift assays (EMSA), chromatin immunoprecipitation (ChIP), or luciferase reporter systems. Researchers use it to investigate diseases linked to oxidative stress dysregulation, including cancer, neurodegenerative disorders, and metabolic syndromes. Additionally, MAFG's involvement in Nrf2-mediated pathways makes it a potential target for drug development, particularly in therapies aiming to modulate antioxidant or detoxification responses. Its recombinant form is critical for high-throughput screening of compounds that influence these pathways, advancing precision medicine and therapeutic innovation.
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