| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | PRKWNK3 |
| WB Predicted band size | 198 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human WNK3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于WNK3抗体的3篇参考文献示例(注：文献信息为模拟，实际引用需核实)：
1. **文献名称**：*"WNK3 kinase regulates neuronal chloride homeostasis and impacts neurological function"*
**作者**：Shekarabi M, et al.
**摘要**：研究利用WNK3特异性抗体在小鼠脑组织中定位WNK3蛋白，发现其通过调控离子共转运蛋白NKCC1/KCC2影响神经元氯离子平衡，提示其在神经系统疾病中的潜在作用。
2. **文献名称**：*"Hypertension-associated kinase WNK3 phosphorylates ion channels in renal tubules"*
**作者**：Kahle KT, et al.
**摘要**：通过WNK3抗体进行免疫沉淀和Western blot分析，揭示WNK3在肾小管上皮细胞中磷酸化钠钾氯共转运蛋白(NKCC2)，参与血压调节及电解质稳态。
3. **文献名称**：*"Antibody-based profiling of WNK3 expression in human tissues reveals organ-specific signaling"*
**作者**：Zagórska A, et al.
**摘要**：开发高特异性WNK3抗体并用于免疫组化，发现WNK3在肾脏、大脑及心脏中差异表达，提示其组织特异性功能及与相关疾病的关联。
(注：以上内容为示例，实际文献需通过PubMed等数据库检索确认。)
The WNK3 antibody is a research tool designed to detect and study WNK3 (With No Lysine [K] 3), a serine/threonine kinase belonging to the WNK family. WNK kinases are unique due to their atypical placement of a catalytic lysine residue, which distinguishes them from conventional kinases. WNK3 is broadly expressed in tissues, including the brain, kidney, and vascular endothelium, and plays a critical role in regulating ion transport and cellular homeostasis. It modulates the activity of ion cotransporters like NKCC1/2 and NCC, influencing electrolyte balance, blood pressure, and cell volume regulation.
Research on WNK3 has linked it to hypertension, neurological disorders, and kidney diseases. Dysregulation of WNK3 signaling may contribute to pathological conditions, such as familial hyperkalemic hypertension (FHHt) or cerebral edema. Antibodies against WNK3 enable scientists to investigate its expression patterns, post-translational modifications, and interactions with other proteins in both physiological and disease contexts. These antibodies are commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to map WNK3 localization and quantify its levels in experimental models.
Recent studies also explore WNK3's role in cancer progression and neuroprotection, expanding its therapeutic relevance. The development of specific WNK3 antibodies has thus been pivotal in unraveling its multifaceted biological functions and potential as a drug target.
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