| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human WNT5B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于WNT5B抗体的参考文献示例(内容为虚构概括，建议通过学术数据库核实具体文献)：
1. **"WNT5B monoclonal antibody inhibits melanoma cell invasion by suppressing Wnt/β-catenin signaling"**
- 作者：Zhang et al. (2017)
- 摘要：研究开发了一种特异性WNT5B单克隆抗体，证实其通过阻断Wnt/β-catenin通路抑制黑色素瘤细胞的侵袭和迁移能力。
2. **"WNT5B expression in gastric cancer and its clinical significance: A study using immunohistochemistry"**
- 作者：Tanaka et al. (2019)
- 摘要：利用WNT5B抗体进行免疫组化分析，发现WNT5B在胃癌组织中高表达，且与患者预后不良显著相关。
3. **"Characterization of a novel anti-WNT5B antibody for functional studies in developmental biology"**
- 作者：Miller et al. (2018)
- 摘要：报道了一种高亲和力WNT5B抗体的开发与验证，成功应用于斑马鱼胚胎模型，揭示WNT5B在胚胎肢体发育中的调控作用。
4. **"Therapeutic targeting of WNT5B in triple-negative breast cancer using a neutralizing antibody"**
- 作者：Patel et al. (2021)
- 摘要：研究表明WNT5B中和抗体可通过抑制非经典Wnt信号通路，降低三阴性乳腺癌细胞的转移和化疗耐药性。
建议通过PubMed或Google Scholar搜索上述关键词，获取真实文献及详细信息。
Wnt5b antibody is a research tool designed to target the Wnt5b protein, a member of the Wnt family of secreted glycoproteins involved in critical biological processes such as embryonic development, tissue homeostasis, and cancer progression. Wnt5b, a paralog of Wnt5a, primarily activates non-canonical Wnt signaling pathways (e.g., planar cell polarity and Wnt/Ca²+ pathways) rather than the canonical β-catenin-dependent pathway. It plays roles in cell migration, polarity, and differentiation, with dysregulation linked to tumorigenesis, metastasis, and poor prognosis in cancers like breast, colorectal, and melanoma.
Wnt5b antibodies are used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect expression patterns, localization, and functional interactions of Wnt5b in cells and tissues. These antibodies aid in studying its role in developmental biology, stem cell regulation, and disease mechanisms, particularly in cancers where Wnt5b overexpression correlates with aggressive phenotypes.
Therapeutic interest in Wnt5b inhibitors is emerging, as blocking its signaling may suppress tumor invasion and metastasis. However, challenges remain in specificity and delivery. Commercially available Wnt5b antibodies vary in clonality, species reactivity, and applications, requiring validation for experimental consistency. Ongoing research aims to clarify Wnt5b's context-dependent roles and explore its potential as a diagnostic or therapeutic target.
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