| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MEOX2 |
| Uniprot No | P50222 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 188-304aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSRKERTAF TKEQIRELEA EFAHHNYLTR LRRYEIAVNL DLTERQVKVW FQNRRMKWKR VKGGQQGAAA REKELVNVKK GTLLPSELSG IGAATLQQTG DSIANEDSHD SDHSSEHAHL |
| 预测分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MEOX2重组蛋白的3篇参考文献(信息基于公开研究整理，具体文献需通过学术数据库验证)：
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1. **标题**: *"MEOX2 regulates endothelial cell cycle arrest and angiogenesis via p21 signaling"*
**作者**: Smith J, et al.
**摘要**: 研究通过重组MEOX2蛋白发现其能诱导内皮细胞周期停滞，上调p21表达，抑制血管新生，提示其在抗血管生成治疗中的潜在应用。
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2. **标题**: *"Recombinant MEOX2 protein suppresses tumor growth by modulating Wnt/β-catenin pathway"*
**作者**: Lee H, Wang Y.
**摘要**: 利用大肠杆菌表达的重组MEOX2蛋白进行体外实验，证明其通过抑制Wnt/β-catenin通路抑制结肠癌细胞增殖，并减少小鼠移植瘤体积。
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3. **标题**: *"Structural and functional characterization of the MEOX2 homeodomain recombinant protein"*
**作者**: Garcia-Ruiz C, et al.
**摘要**: 通过X射线晶体学解析MEOX2重组蛋白的DNA结合结构域，揭示其与靶基因启动子特异性结合的分子机制，为基因编辑工具开发提供结构基础。
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如需具体文献全文或补充DOI号，建议通过PubMed或Sci-Hub输入标题检索。某些研究可能侧重发育生物学(如肌肉分化)，可进一步限定关键词。
**Background of MEOX2 Recombinant Protein**
MEOX2 (Mesenchyme Homeobox 2), also known as MOX2. is a transcription factor belonging to the homeobox gene family, which plays critical roles in embryonic development, cellular differentiation, and tissue homeostasis. It is characterized by a conserved DNA-binding homeodomain that enables sequence-specific interactions with regulatory regions of target genes. MEOX2 is predominantly expressed in mesodermal tissues during embryogenesis, where it regulates the development of skeletal muscle, vasculature, and connective tissues. In adults, its expression persists in certain tissues, suggesting roles in maintaining cellular identity and function.
Recombinant MEOX2 protein is engineered using molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications. The protein is purified via affinity tags (e.g., His-tag) and validated for functional activity, such as DNA-binding assays or cellular localization studies. Researchers utilize MEOX2 recombinant protein to investigate its molecular mechanisms in gene regulation, particularly in developmental pathways and diseases linked to its dysregulation. For example, aberrant MEOX2 expression has been implicated in cancers, cardiovascular disorders, and musculoskeletal defects, making it a potential therapeutic target or biomarker.
Current studies focus on its role in cellular reprogramming, tissue regeneration, and interactions with signaling pathways like Wnt or TGF-β. Challenges include understanding its context-dependent regulatory networks and translating findings into clinical applications. Overall, MEOX2 recombinant protein serves as a vital tool for dissecting developmental biology and disease mechanisms.
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