| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CT100; PESCRG1; ECAT15-2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human DPPA2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DPPA2抗体的3篇参考文献的简要信息：
1. **文献名称**：*DPPA2/4 as a trigger of signaling pathways through antibody-recognized surface antigens in pluripotent stem cells*
**作者**：Madison JM等
**摘要**：研究揭示了DPPA2和DPPA4蛋白在多能干细胞表面抗原中的表达，通过特异性抗体识别，发现其参与调控Wnt和FGF信号通路，影响干细胞的自我更新与分化。
2. **文献名称**：*Dynamic expression of DPPA2 during early human embryogenesis and pluripotency regulation*
**作者**：Kim J等
**摘要**：利用DPPA2抗体进行免疫组化和流式分析，发现其在人类早期胚胎细胞中高表达，且与OCT4等核心多能性因子共定位，提示其在维持胚胎干细胞未分化状态中的关键作用。
3. **文献名称**：*Characterization of a novel monoclonal antibody against DPPA2 for epigenetic studies in cancer*
**作者**：Tanaka R等
**摘要**：报道了一种针对DPPA2表位的新型单克隆抗体的开发，验证了其在染色质免疫沉淀(ChIP)和免疫印迹中的高特异性，并发现DPPA2在多种癌细胞中异常表达，可能与表观遗传失调相关。
**Background of DPPA2 Antibody**
DPPA2 (Developmental Pluripotency-Associated 2) is a DNA-binding protein predominantly expressed in early embryonic development and germ cells, playing a critical role in maintaining pluripotency and regulating epigenetic states in embryonic stem cells (ESCs). It is localized to chromosome 3q13.13 in humans and contains conserved structural domains, including a SAP motif (involved in chromatin interaction) and a zinc finger-like domain. DPPA2 interacts with other pluripotency factors, such as OCT4 and NANOG, to modulate gene expression networks essential for self-renewal and lineage specification.
Antibodies targeting DPPA2 are vital tools for studying its expression, localization, and function in pluripotency and differentiation. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and flow cytometry to analyze DPPA2 dynamics during early embryogenesis, germ cell development, and reprogramming. Due to DPPA2’s low expression in somatic cells and transient activation during reprogramming, specific and high-affinity antibodies are crucial to avoid cross-reactivity with related proteins.
DPPA2 dysregulation is implicated in germ cell tumors and teratomas, highlighting its clinical relevance. However, antibody validation remains challenging, requiring knockout cell lines or siRNA controls to confirm specificity. Overall, DPPA2 antibodies serve as essential reagents for unraveling mechanisms of pluripotency, epigenetic regulation, and disease, with potential applications in regenerative medicine and cancer research.
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