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Human mammary carcinoma marker CA15-3 (CA15-3) ELISA kit

  • 中文名： 人乳腺癌标志物CA15-3(CA15-3)ELISA Kit
货号: IPD-E04772h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人乳腺癌标志物CA15-3(CA15-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清样本中的CA15-3含量。CA15-3是一种与乳腺癌相关的肿瘤标志物。正常情况下，人体含量较低。，当乳腺发生癌变时，癌细胞会产生并释放CA15-3到血液中，使其含量升高，因此可通过检测它辅助乳腺癌的诊断、病情监测等。试剂盒检测范围为15 U/ml- 250 U/mL，灵敏度为3.8 U/mL。可支持乳腺癌基础研究、药物开发中的生物标志物动态分析以及癌症相关分子机制研究，为实验室提供稳定可靠的定量检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    ADMCKD ELISA Kit; ADMCKD1 ELISA Kit; Breast carcinoma associated antigen DF3 ELISA Kit; Breast carcinoma-associated antigen DF3 ELISA Kit; CA 15-3 ELISA Kit; CA15 3 ELISA Kit; CA15 3 antigen ELISA Kit; CA15-3 ELISA Kit; CA15.3 ELISA Kit; Cancer antigen 15-3 ELISA Kit; Carcinoma associated mucin ELISA Kit; Carcinoma-associated mucin ELISA Kit; CD 227 ELISA Kit; CD227 ELISA Kit; DF3 antigen ELISA Kit; EMA ELISA Kit; Episialin ELISA Kit; Epithelial Membrane Antigen ELISA Kit; H23 antigen ELISA Kit; H23AG ELISA Kit; KL 6 ELISA Kit; KL-6 ELISA Kit; KL6 ELISA Kit; Krebs von den Lungen-6 ELISA Kit; MAM 6 ELISA Kit; MAM6 ELISA Kit; MCD ELISA Kit; MCKD ELISA Kit; MCKD1 ELISA Kit; Medullary cystic kidney disease 1 (autosomal dominant) ELISA Kit; Medullary cystic kidney disease, autosomal dominant ELISA Kit; MUC 1 ELISA Kit; MUC-1 ELISA Kit; MUC-1/SEC ELISA Kit; MUC-1/X ELISA Kit; MUC1 ELISA Kit; MUC1-alpha ELISA Kit; MUC1-beta ELISA Kit; MUC1-CT ELISA Kit; MUC1-NT ELISA Kit; MUC1/ZD ELISA Kit; MUC1_HUMAN ELISA Kit; Mucin 1 ELISA Kit; Mucin 1 cell surface associated ELISA Kit; Mucin 1 transmembrane ELISA Kit; Mucin 1, cell surface associated ELISA Kit; Mucin-1 subunit beta ELISA Kit; Peanut reactive urinary mucin ELISA Kit; Peanut-reactive urinary mucin ELISA Kit; PEM ELISA Kit; PEMT ELISA Kit; Polymorphic epithelial mucin ELISA Kit; PUM ELISA Kit; Tumor associated epithelial membrane antigen ELISA Kit; Tumor associated epithelial mucin ELISA Kit; Tumor associated mucin ELISA Kit; Tumor-associated epithelial membrane antigen ELISA Kit; Tumor-associated mucin ELISA Kit
  • 缩写：
    CA15-3
  • Uniprot No.：
    P15941
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum
  • 检测范围：
    15 U/mL - 250 U/mL
  • 灵敏度：
    3.8 U/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<15%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<20%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human CA15-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    90


    Range %

    86-99


    1:2

    Average %

    97


    Range %

    91-105


    1:4

    Average %

    95


    Range %

    89-100


    1:8

    Average %

    92


    Range %

    88-98


  • 回收率：

    The recovery of human CA15-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    97

    93-99


    EDTA plasma (n=4)

    96

    90-101


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.








    U/ml

    OD1

    OD2

    Average



    0

    0.109

    0.114

    0.112



    15

    0.187

    0.196

    0.192



    40

    0.425

    0.401

    0.413



    100

    0.964

    0.975

    0.970



    250

    1.937

    1.836

    1.887




  • 本试剂盒所含材料：



    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human CA15-3 antibody.

    • Five vials standard (0.5 ml/bottle)--- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial HRP-conjugated CA15-3 antibody (6 ml/bottle) --- Bind to the CEA in the samples or standards and react with the substrate to make the solution chromogenic.

    • One vial Sample Diluent (11ml/bottle) ---Dilute the sample solution.

    • One vial Wash Buffer (20x concentrate) (15 ml/bottle) --- Wash away unbound or free substances.

    • One vial Substrate A (7ml/bottle) --- Mix with substrate B and then interact with TMB to elicit a chromogenic reaction.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at603 nm or 630 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days


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