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Human von Willebrand Factor,vWF ELISA Kit

  • 中文名： 人血管性血友病因子/瑞斯托霉素辅因子(VWF)ELISA Kit
货号: IPD-E08437h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人血管性血友病因子/瑞斯托霉素辅因子(VWF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的VWF含量。VWF（血管性血友病因子）是一种多聚糖蛋白，在止血过程中至关重要。其与血小板结合可促进血栓形成，还能保护凝血因子Ⅷ。相关研究围绕其结构、功能及与疾病关联机制展开，如在血管性血友病等疾病中VWF异常，这些研究为治疗提供了方向。试剂盒检测范围为6.25 ng/ml-400 ng/ml.，灵敏度为1.56 ng/ml.。该产品适用于基础医学研究、疾病机制探索、药物作用评估等科研场景，例如探究心血管疾病动物模型中VWF表达动态，或体外评估药物对血管内皮细胞VWF分泌的影响，为凝血功能相关研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Coagulation factor VIII ELISA Kit; Coagulation factor VIII VWF ELISA Kit; F8VWF ELISA Kit; Factor VIII related antigen ELISA Kit; von Willebrand antigen 2 ELISA Kit; von Willebrand antigen II ELISA Kit; Von Willebrand disease ELISA Kit; Von Willebrand factor precursor ELISA Kit; VWD ELISA Kit; vWF ELISA Kit; VWF_HUMAN ELISA Kit
  • 缩写：
    VWF
  • Uniprot No.：
    P04275
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    6.25 ng/ml-400 ng/ml.
  • 灵敏度：
    1.56 ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    86


    Range %

    82-92


    1:2

    Average %

    89


    Range %

    85-96


    1:4

    Average %

    100


    Range %

    95-105


    1:8

    Average %

    89


    Range %

    82-94


  • 回收率：

    The recovery of human vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    96

    91-100


    EDTA plasma (n=4)

    94

    90-97


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    400

    2.586

    2.556

    2.571

    2.407


    200

    1.785

    1.799

    1.792

    1.628


    100

    1.186

    1.153

    1.170

    1.006


    50

    0.700

    0.721

    0.711

    0.547


    25

    0.496

    0.473

    0.485

    0.321


    12.5

    0.382

    0.352

    0.367

    0.203


    6.25

    0.290

    0.273

    0.282

    0.118


    0

    0.161

    0.167

    0.164




  • 货期：
    3-5 working days

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