Rabbit Polyclonal MYL12B Antibody

The MYL12B antibody targets myosin light chain 12B (MYL12B), a regulatory component of non-muscle myosin II (NMII) complexes. MYL12B, encoded by the MYL12B gene, is one of three isoforms (MYL12A, MYL12B, MYL12C) generated via alternative splicing of the MYL12 transcript. It plays a critical role in regulating actomyosin contractility, which governs essential cellular processes such as cytokinesis, cell migration, and maintenance of cell shape. MYL12B specifically binds to the heavy chain of NMIIA (MYH9), modulating its ATPase activity and mechanical functions. Dysregulation of MYL12B has been implicated in pathological conditions, including cancer metastasis, cardiovascular diseases, and neurological disorders, due to its influence on cytoskeletal dynamics. Researchers employ MYL12B antibodies in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study its expression, localization, and interaction partners in cellular and tissue contexts. These antibodies are also used to investigate phosphorylation events (e.g., at Ser19/Thr18) that activate myosin light chain kinase pathways. Commercial MYL12B antibodies are typically validated for specificity against its unique C-terminal sequence, distinguishing it from the MYL12A isoform. Its conserved structure across vertebrates makes it a valuable tool in comparative studies of cell mechanics and developmental biology.