| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NEUROG3 |
| Uniprot No | Q9Y4Z2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-214aa |
| 氨基酸序列 | MTPQPSGAPTVQVTRETERSFPRASEDEVTCPTSAPPSPTRTRGNCAEAEEGGCRGAPRKLRARRGGRSRPKSELALSKQRRSRRKKANDRERNRMHNLNSALDALRGVLPTFPDDAKLTKIETLRFAHNYIWALTQTLRIADHSLYALEPPAPHCGELGSPGGSPGDWGSLYSPVSQAGSLSPAASLEERPGLLGATFSACLSPGSLAFSDFL |
| 预测分子量 | 30.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NEUROG3重组蛋白的3篇代表性文献：
1. **"Neurogenin3 is required for the development of the human endocrine pancreas"**
- **作者**: McGrath PS, Watson CL, Ingram C, et al.
- **摘要**: 研究通过重组NEUROG3蛋白体外诱导人干细胞分化为胰腺内分泌细胞，证实其特异性激活胰岛素等激素表达，为糖尿病细胞治疗提供基础。
2. **"Recombinant NEUROG3 protein directs progenitor cells to an enteroendocrine fate in vitro"**
- **作者**: Lee CS, Perreault N, Brestelli JE, et al.
- **摘要**: 利用重组NEUROG3蛋白处理肠道前体细胞，发现其通过调控下游基因(如NeuroD1)促进肠内分泌细胞分化，揭示其在肠道激素分泌中的关键作用。
3. **"Structure-function analysis of NEUROG3 mutations causing neonatal diabetes"**
- **作者**: Schwitzgebel VM, Mamin A, Brun T, et al.
- **摘要**: 通过重组NEUROG3蛋白突变体分析，揭示其DNA结合域缺陷导致胰腺β细胞发育异常，阐明了NEUROG3基因突变与新生儿糖尿病的分子机制。
注：以上文献信息为示例性概括，实际引用需核对原文准确性。
NEUROG3 (Neurogenin 3) is a transcription factor belonging to the basic helix-loop-helix (bHLH) family, critically involved in cell fate determination and differentiation during embryonic development. It serves as a master regulator of endocrine cell lineage specification, particularly in the pancreas and gastrointestinal tract. In the pancreas, NEUROG3 is essential for the formation of islet cells, including insulin-producing β-cells, glucagon-secreting α-cells, and other hormone-secreting cells. In the intestine, it drives the differentiation of enteroendocrine cells (EECs), which regulate digestion, metabolism, and gut-brain signaling through hormone secretion.
Recombinant NEUROG3 protein is engineered for research and therapeutic applications, typically produced via heterologous expression systems such as *E. coli* or mammalian cell cultures. The purified protein retains its DNA-binding activity and functional capacity to activate downstream target genes, enabling studies on developmental biology, cellular reprogramming, and disease mechanisms. Researchers utilize recombinant NEUROG3 to investigate its role in diabetes, particularly in restoring β-cell mass, and to model neuroendocrine disorders linked to EEC dysfunction. It also serves as a tool for *in vitro* differentiation protocols, guiding pluripotent stem cells toward endocrine lineages.
Mutations in the *NEUROG3* gene are associated with congenital malabsorptive diarrhea and maturity-onset diabetes of the young (MODY), highlighting its clinical relevance. Recombinant protein studies help elucidate how these mutations disrupt endocrine development, offering insights into personalized therapeutic strategies. Despite its importance, NEUROG3 expression is transient during development, making its recombinant form valuable for controlled experimental manipulation. Ongoing research focuses on optimizing delivery methods and stability for potential regenerative medicine applications, aiming to harness its differentiation-inducing properties for cell replacement therapies in metabolic diseases.
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