| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/45-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RFP; RNF76 |
| WB Predicted band size | 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human TRIM27 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于TRIM27抗体的参考文献及其摘要概括(基于真实研究背景模拟，非真实文献)：
1. **文献名称**：TRIM27 modulates ubiquitination dynamics to regulate DNA repair pathways
**作者**：Smith J, et al.
**摘要**：该研究利用TRIM27特异性抗体进行免疫共沉淀(Co-IP)和蛋白质印迹(Western blot)，揭示了TRIM27通过泛素化修饰调控DNA损伤修复蛋白的稳定性，影响细胞对电离辐射的敏感性。
2. **文献名称**：TRIM27 promotes tumor progression by stabilizing oncogenic kinases in lung adenocarcinoma
**作者**：Chen L, et al.
**摘要**：通过TRIM27抗体进行免疫组化(IHC)分析临床样本，发现TRIM27在肺癌组织中高表达，并通过泛素-蛋白酶体途径稳定致癌激酶EGFR，促进肿瘤生长和转移。
3. **文献名称**：TRIM27 negatively regulates antiviral innate immunity by targeting MAVS for degradation
**作者**：Wang Y, et al.
**摘要**：研究使用TRIM27抗体进行免疫荧光和流式细胞术，证明TRIM27通过介导MAVS蛋白的泛素化降解，抑制宿主抗病毒免疫应答，为病毒感染机制提供新见解。
4. **文献名称**：TRIM27 deficiency enhances neuronal autophagy and mitigates neurotoxicity in Parkinson’s disease models
**作者**：Kim S, et al.
**摘要**：利用TRIM27敲除小鼠模型及抗体进行脑组织免疫染色，发现TRIM27通过调控自噬相关蛋白LC3的泛素化，影响帕金森病中α-突触核蛋白的清除效率。
注：以上内容为基于TRIM27相关研究领域的典型方向模拟，实际文献需通过PubMed或Google Scholar以“TRIM27 antibody”为关键词检索。
The TRIM27 antibody is a crucial tool for studying the TRIpartite Motif-containing protein 27 (TRIM27), a member of the TRIM/RBCC family characterized by RING, B-box, and coiled-coil domains. TRIM27. also known as Ret finger protein (RFP), functions as an E3 ubiquitin ligase, regulating protein degradation, transcriptional modulation, and immune signaling. It plays roles in diverse cellular processes, including apoptosis, autophagy, and antiviral responses, and has been implicated in cancer progression, neurodevelopmental disorders, and autoimmune diseases.
TRIM27 antibodies are primarily used to detect and quantify TRIM27 expression in various experimental models (e.g., cell lines, tissues) through techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies aid in elucidating TRIM27's interaction networks, post-translational modifications (e.g., ubiquitination), and subcellular localization. Research utilizing TRIM27 antibodies has revealed its oncogenic potential in cancers (e.g., gastric, lung) by promoting cell proliferation and metastasis, as well as its regulatory role in immune pathways like NF-κB and type I interferon signaling.
Validated TRIM27 antibodies are essential for exploring its dual roles in tumor suppression and progression, depending on cellular context. Their specificity and sensitivity are critical for distinguishing TRIM27 isoforms and avoiding cross-reactivity with homologous TRIM proteins. Ongoing studies focus on TRIM27's therapeutic potential, making its antibody a key reagent in molecular and clinical research.
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