| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CIA2A; FAM96A |
| WB Predicted band size | 18 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human CIAO2A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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由于目前公开的学术数据库中尚未能查询到明确以"CIAO2A抗体"为核心研究对象的文献，以下提供模拟的参考文献格式示例供参考(请注意这些为虚构示例，实际文献需通过学术数据库验证)：
1. **《CIAO2A在DNA损伤修复中的功能研究》**
- 作者：Smith J, et al.
- 摘要：本文通过开发特异性CIAO2A抗体，证实CIAO2A蛋白通过与BRCA1复合物相互作用参与同源重组修复，缺失时导致基因组不稳定性增加。
2. **《CIAO2A抗体在结直肠癌诊断中的应用》**
- 作者：Wang L, et al.
- 摘要：研究验证了CIAO2A抗体的高特异性，发现CIAO2A在结直肠癌组织中表达上调，可能作为预后生物标志物。
3. **《CIAO2A蛋白的亚细胞定位与功能》**
- 作者：Garcia R, et al.
- 摘要：利用免疫荧光和CIAO2A抗体，发现该蛋白主要定位于细胞核，调控细胞周期G1/S转换，干扰其表达引发细胞周期停滞。
**建议检索策略**：
1. 在PubMed/Google Scholar中使用关键词：`"CIAO2A" AND (antibody OR expression)`
2. 结合可能的别名：如`"CIAO2A" 可能为 "Ciapin2"`或与`"iron-sulfur cluster assembly"`相关，可尝试扩展搜索
3. 查阅抗体公司产品说明(如Abcam、Sigma)，获取引用文献
如需进一步协助文献筛选，请提供更具体的研究背景或目标方向。
**Background of CIAO2A Antibody**
The CIAO2A (Cytosolic Iron-Sulfur Assembly Component 2A) antibody is a tool used to study the CIAO2A protein, a key component of the cytosolic iron-sulfur cluster assembly (CIA) machinery. Iron-sulfur (Fe-S) clusters are essential cofactors for numerous enzymes involved in critical cellular processes, including DNA repair, metabolic pathways, and redox regulation. The CIA pathway, localized in the cytosol and nucleus, is responsible for assembling and inserting Fe-S clusters into target apoproteins. CIAO2A, along with CIAO1 and MMS19. forms the CIA2A-MMS19-CIAO1 complex, which facilitates the transfer of Fe-S clusters to specific client proteins.
CIAO2A is particularly crucial for the maturation of enzymes like DNA polymerases and helicases, linking Fe-S cluster biogenesis to genome stability and cell cycle progression. Dysregulation of CIAO2A has been implicated in diseases such as cancer and neurodegenerative disorders, where Fe-S cluster metabolism is often compromised.
The CIAO2A antibody enables researchers to detect and quantify CIAO2A expression in various tissues and cell lines using techniques like Western blotting, immunofluorescence, or immunohistochemistry. It aids in exploring the protein’s role in Fe-S cluster assembly, its interaction partners, and its broader implications in cellular homeostasis and disease mechanisms. Studies utilizing this antibody contribute to understanding how disruptions in Fe-S biogenesis impact human health, offering potential therapeutic insights.
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