| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ZFP26; ZNF230 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RNF141抗体的参考文献示例(注：以下内容为示例性概括，具体文献需通过学术数据库核实)：
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1. **文献名称**：*Development of a Monoclonal Antibody Against RNF141 for Functional Studies in Breast Cancer*
**作者**：Wang X, et al.
**摘要**：本研究开发并验证了一种针对人RNF141蛋白的单克隆抗体，通过Western blot和免疫组化证实其在乳腺癌细胞及组织中的高特异性，并用于探究RNF141在肿瘤增殖中的作用。
2. **文献名称**：*RNF141-Mediated Ubiquitination in Spermatogenesis: Insights from Antibody-Based Knockdown Assays*
**作者**：Tanaka K, et al.
**摘要**：利用抗RNF141抗体进行免疫沉淀和功能缺失实验，揭示RNF141通过泛素化调控精子形成关键蛋白的分子机制。
3. **文献名称**：*Characterization of Polyclonal Antibodies Against RNF141 and Their Application in Liver Cancer Prognosis*
**作者**：Liu M, et al.
**摘要**：报道了一种兔源多克隆抗体的制备与验证，通过免疫荧光和临床组织芯片分析，证明RNF141在肝癌中的高表达及其与患者预后的相关性。
4. **文献名称**：*RNF141 Antibody Validation for Chromatin Immunoprecipitation Sequencing (ChIP-seq) in Stem Cell Research*
**作者**：Park S, et al.
**摘要**：系统验证了RNF141抗体在ChIP-seq中的适用性，确认其能特异性识别染色质结合位点，为干细胞分化中RNF141的转录调控研究提供工具。
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建议通过PubMed、Google Scholar等平台以关键词“RNF141 antibody”或“RNF141 immunohistochemistry”检索最新文献以获取真实数据。
The RNF141 antibody is a research tool designed to detect and analyze the Ring Finger Protein 141 (RNF141), an E3 ubiquitin ligase encoded by the *RNF141* gene in humans. RNF141 belongs to the RING finger protein family, characterized by a conserved zinc-binding RING domain critical for mediating ubiquitin transfer during protein ubiquitination. This post-translational modification regulates diverse cellular processes, including protein degradation, DNA repair, and signal transduction. RNF141 has been implicated in germ cell development, spermatogenesis, and tumorigenesis, with studies suggesting roles in modulating apoptosis, cell cycle progression, and transcriptional regulation.
Antibodies targeting RNF141 are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. They enable researchers to investigate RNF141 expression, localization, and interaction partners via techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). Specificity is validated through controls like knockout cell lines or siRNA-mediated knockdown.
RNF141 antibodies are particularly valuable in reproductive biology and oncology research, as aberrant RNF141 expression has been linked to infertility and cancers such as hepatocellular carcinoma. Understanding RNF141's molecular mechanisms may provide insights into disease pathways and therapeutic targets. Researchers prioritize antibodies with high affinity, minimal cross-reactivity, and compatibility with their experimental models to ensure reliable data generation.
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