首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠载脂蛋白A1(Apo-A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液、组织匀浆样本中的APOA1含量。APOA1是重要的载脂蛋白。它在脂质转运、胆固醇逆向转运中起关键作用，与动脉粥样硬化等心血管疾病密切相关。研究中，提升其水平或功能、改善其相关代谢机制等，是探索心血管疾病防治新策略的重要方向。试剂盒检测范围为31.25 ng/mL-2000 ng/mL，本产品可满足实验室对脂代谢相关疾病动物模型研究、药物干预效果评价、细胞水平脂蛋白合成机制探索等科研需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Apoa1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit
缩写：APOA1
Uniprot No.：Q00623
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, urine, tissue homogenates
检测范围：31.25 ng/mL-2000 ng/mL
灵敏度：7.81 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:2000 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:4000 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:8000 | Average % | 97 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of mouse Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 93-104 | ||||
| EDTA plasma (n=4) | 99 | 95-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected20002.2142.2562.2352.06210001.8561.8901.8731.7005001.4611.4001.4311.2582500.9640.9990.9820.8091250.6890.6720.6810.50862.50.4810.4910.4860.31331.250.3500.3680.3590.18600.1720.1740.173 | ||||||
货期：3-5 working days
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