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产品描述：猪载脂蛋白A1(Apo-A1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOA1含量。APOA1是一种位于细胞膜外的分泌蛋白，是高密度脂蛋白（HDL）的主要结构载脂蛋白，在逆向胆固醇转运途径中起关键作用。其与细胞表面受体SR-BI相互作用，促进胆固醇从组织到肝脏的逆向运输，具有抗炎、抗动脉粥样硬化等作用。研究显示，APOA1与病毒感染、动脉粥样硬化、糖尿病、神经系统疾病、肿瘤等疾病有关。试剂盒检测范围为80 μg/mL-2000 μg/mL，适用于猪脂代谢机制研究、心血管疾病动物模型评估、营养干预对脂蛋白代谢的影响分析等科研领域，为猪相关代谢性疾病的基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：APOA1Apolipoprotein A-I ELISA Kit; Apo-AI ELISA Kit; ApoA-I ELISA Kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA Kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA Kit
缩写：APOA1
Uniprot No.：P18648
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：80 μg/mL-2000 μg/mL
灵敏度：50 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 89-103 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-103 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-100 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 82-97 | |||||
回收率：
| The recovery of pig Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 96-104 | ||||
| EDTA plasma (n=4) | 90 | 86-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average20000.3420.3480.3458000.5330.4830.5084800.6820.6570.6702001.0210.9871.004801.2671.3251.29601.9031.9541.929 | ||||||
货期：3-5 working days
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