首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠骨成型蛋白7(BMP-7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的BMP7含量。BMP7 即骨形态发生蛋白 7，它属于 TGF-β 超家族成员。在骨骼发育和再生中发挥关键作用，能诱导骨和软骨形成。研究表明其机制涉及激活相关信号通路，调节细胞增殖与分化，还在肾脏保护等方面有潜在作用，有广泛研究前景。试剂盒检测范围为0.625 pg/mL-40 pg/mL，可广泛应用于骨骼再生机制、细胞分化调控、肾脏疾病模型或代谢相关疾病的分子机制研究。科研人员可通过分析体外细胞模型中BMP - 7的分泌动态，或评估动物疾病模型中蛋白表达水平的变化，为组织工程、再生医学及病理生理学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Bmp7 ELISA Kit; Bmp-7 ELISA Kit; Op1 ELISA Kit; Bone morphogenetic protein 7 ELISA Kit; BMP-7 ELISA Kit; Osteogenic protein 1 ELISA Kit; OP-1 ELISA Kit
缩写：BMP7
Uniprot No.：P23359
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.625 pg/mL-40 pg/mL
灵敏度：0.156 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-92 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 98-106 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 97-103 | |||||
回收率：
| The recovery of mouse BMP-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-96 | ||||
| EDTA plasma (n=4) | 95 | 93-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected402.4492.4402.4452.295201.7881.8181.8031.653101.1961.1811.1891.03950.5840.6040.5940.4442.50.4240.4150.4200.2701.250.3210.3120.3170.1670.6250.2720.2550.2640.11400.1520.1480.150 | ||||||
货期：3-5 working days
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