| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MRL3; RPML3; COXPD9 |
| WB Predicted band size | 39 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MRPL3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MRPL3抗体的3篇参考文献示例(注：文献为虚拟示例，实际引用需通过学术数据库核实)：
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1. **文献名称**：*MRPL3 is essential for mitochondrial ribosome assembly and respiratory chain function*
**作者**：Smith A, et al.
**摘要**：该研究通过CRISPR敲除MRPL3基因，发现其缺失导致线粒体核糖体大亚基组装缺陷，并显著降低呼吸链复合物活性。利用MRPL3抗体进行免疫印迹和免疫荧光，证实其在HeLa细胞线粒体中的定位，提示MRPL3对线粒体蛋白质合成的关键作用。
2. **文献名称**：*Overexpression of MRPL3 correlates with poor prognosis in hepatocellular carcinoma*
**作者**：Zhang Y, et al.
**摘要**：通过免疫组化(使用MRPL3抗体)分析肝癌组织样本，发现MRPL3在肿瘤组织中高表达，且与患者总生存期缩短相关。功能实验表明，抑制MRPL3可降低癌细胞增殖，提示其作为潜在治疗靶点。
3. **文献名称**：*A novel MRPL3 mutation causes mitochondrial encephalomyopathy in humans*
**作者**：Lee S, et al.
**摘要**：研究报道一例由MRPL3基因错义突变引起的线粒体脑肌病病例。通过Western blot(使用MRPL3抗体)发现患者成纤维细胞中MRPL3蛋白水平显著下降，导致线粒体翻译缺陷和ATP合成减少，为疾病机制提供分子证据。
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**提示**：实际研究中，建议通过PubMed或Google Scholar以“MRPL3 antibody”或“MRPL3 mitochondrial ribosome”为关键词检索最新文献，并优先选择近5年、高影响因子期刊的研究。
The MRPL3 (Mitochondrial Ribosomal Protein L3) antibody is a valuable tool in studying mitochondrial protein synthesis and its associated cellular processes. MRPL3 is a core component of the large subunit of the mitochondrial ribosome, essential for mitochondrial translation machinery. Mitochondria, the energy-producing organelles, rely on their own ribosomes to synthesize subunits of the electron transport chain (ETC) complexes, which are critical for oxidative phosphorylation. MRPL3 plays a structural and functional role in assembling the mitochondrial ribosome, ensuring proper ETC complex formation and cellular energy homeostasis.
Antibodies targeting MRPL3 are widely used to investigate mitochondrial ribosome biogenesis, protein expression levels, and subcellular localization via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Research involving MRPL3 has linked its dysregulation to mitochondrial disorders, metabolic diseases, and cancer, as disrupted mitochondrial translation can impair energy production and promote tumorigenesis. Studies also explore MRPL3’s role in apoptosis, aging, and neurodegenerative diseases like Parkinson’s, where mitochondrial dysfunction is a hallmark.
Commercial MRPL3 antibodies are typically raised against specific epitopes of human MRPL3. with cross-reactivity tested in common model organisms. Validated antibodies aid in distinguishing mitochondrial vs. cytosolic ribosomal proteins, providing insights into organelle-specific translation mechanisms. As mitochondrial research expands, MRPL3 antibodies remain critical for unraveling the molecular basis of diseases tied to energy metabolism and cellular stress responses.
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