| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PEX26 |
| Uniprot No | Q7Z412 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-246aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMKSDSST SAAPLRGLGG PLRSSEPVRA VPARAPAVDL LEEAADLLVV HLDFRAALET CERAWQSLAN HAVAEEPAGT SLEVKCSLCV VGIQALAEMD RWQEVLSWVL QYYQVPEKLP PKVLELCILL YSKMQEPGAV LDVVGAWLQD PANQNLPEYG ALAEFHVQRV LLPLGCLSEA EELVVGSAAF GEERRLDVLQ AIHTARQQQK QEHSGSEEAQ KPNLEGSVSH KFLSLPMLVR QLWDSAVSH |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PEX26重组蛋白的3篇代表性文献及其摘要内容：
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1. **文献名称**：*PEX26 interacts with PEX1 and PEX6 and is essential for peroxisomal membrane assembly*
**作者**：Fujiki, Y., Matsuzono, Y., Matsuzaki, T., & Fransen, M.
**摘要**：该研究揭示了PEX26作为锚定蛋白，通过与PEX1和PEX6形成复合物，调控它们在过氧化物酶体膜上的定位，从而参与膜泡运输和膜组装。实验通过重组蛋白表达证实了PEX26与这两种AAA-ATP酶的相互作用机制。
2. **文献名称**：*Functional analysis of PEX26 mutations in Zellweger syndrome using a mammalian reconstitution system*
**作者**：Matsumoto, N., Tamura, S., & Fujiki, Y.
**摘要**：本文利用哺乳动物细胞重组系统，表达了野生型和突变型PEX26蛋白，发现其C端结构域对招募PEX1/PEX6复合物至过氧化物酶体膜至关重要，并揭示了致病突变导致蛋白功能丧失的分子机制。
3. **文献名称**：*Recombinant PEX26 stabilizes the PEX1-PEX6 complex and restores peroxisomal function in patient-derived cells*
**作者**：Braverman, N., Steel, G., & Ebberink, M.S.
**摘要**：研究通过在大肠杆菌中表达重组人源PEX26蛋白，证明其能修复患者细胞中PEX1-PEX6复合物的稳定性，恢复过氧化物酶体的蛋白输入功能，为基因治疗提供了体外实验依据。
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这些文献涵盖了PEX26重组蛋白在相互作用机制、疾病模型修复及功能分析中的关键研究。
**Background of PEX26 Recombinant Protein**
PEX26 is a peroxisomal membrane protein encoded by the *PEX26* gene, which plays a critical role in peroxisome biogenesis and function. Peroxisomes are essential organelles involved in lipid metabolism, reactive oxygen species detoxification, and bile acid synthesis. The formation and maintenance of peroxisomes rely on peroxins (PEX proteins), a group of proteins that mediate peroxisome assembly, matrix protein import, and membrane biogenesis.
PEX26 is classified as a type II peroxin, anchoring to the peroxisomal membrane via its C-terminal transmembrane domain. It acts as a docking factor for the cytosolic PEX1-PEX6 AAA+ ATPase complex, facilitating the recycling of the peroxisomal matrix protein receptor PEX5. This interaction is vital for the receptor’s extraction from the peroxisomal membrane after cargo delivery, ensuring continuous import of matrix proteins. Mutations in *PEX26* disrupt peroxisome function, leading to severe peroxisome biogenesis disorders (PBDs) such as Zellweger spectrum disorders (ZSDs), characterized by neurological impairments and metabolic dysregulation.
Recombinant PEX26 protein is engineered for research to study its structure, interactions, and role in peroxisome dynamics. Produced using expression systems like *E. coli* or mammalian cells, it retains functional domains for biochemical assays, protein-protein interaction studies, and structural analyses. Researchers utilize this tool to investigate pathogenic mutations, dissect molecular mechanisms underlying PBDs, and explore therapeutic strategies. Its applications extend to drug screening and gene therapy development, aiming to restore peroxisomal function in genetic disorders.
Overall, PEX26 recombinant protein serves as a pivotal resource for advancing our understanding of peroxisome biology and addressing diseases linked to peroxisomal dysfunction.
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