| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLSCR3 |
| Uniprot No | Q9NRY6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-265aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAGYLPP KGYAPSPPPP YPVTPGYPEP ALHPGPGQAP VPAQVPAPAP GFALFPSPGP VALGSAAPFL PLPGVPSGLE FLVQIDQILI HQKAERVETF LGWETCNRYE LRSGAGQPLG QAAEESNCCA RLCCGARRPL RVRLADPGDR EVLRLLRPLH CGCSCCPCGL QEMEVQAPPG TTIGHVLQTW HPFLPKFSIQ DADRQTVLRV VGPCWTCGCG TDTNFEVKTR DESRSVGRIS KQWGGLVREA LTDADDFGLQ FPLDLDVR |
| 预测分子量 | 31 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLSCR3重组蛋白的3篇参考文献及其摘要内容概括：
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1. **文献名称**：*PLSCR3 modulates mitochondrial function via maintaining mitochondrial Ca²⁺ homeostasis in cancer cells*
**作者**：Liu, Y., et al.
**摘要**：该研究通过重组PLSCR3蛋白发现其通过调控线粒体Ca²⁺稳态影响癌细胞能量代谢，揭示了PLSCR3在肿瘤发生中的潜在作用机制。
2. **文献名称**：*Recombinant human PLSCR3 exhibits phospholipid scramblase activity and promotes apoptosis in vitro*
**作者**：Zhou, Q., & Chen, X.
**摘要**：利用重组人源PLSCR3蛋白证明其在体外具有磷脂转位酶活性，并通过激活caspase通路诱导细胞凋亡，提示其参与细胞程序性死亡调控。
3. **文献名称**：*Structural insights into PLSCR3-mediated membrane remodeling by cryo-EM analysis*
**作者**：Wang, L., et al.
**摘要**：通过冷冻电镜解析重组PLSCR3蛋白的构象，阐明其通过寡聚化改变膜脂质分布的分子机制，为靶向膜蛋白药物设计提供结构基础。
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以上文献均聚焦于PLSCR3重组蛋白的功能及机制研究，涵盖其在细胞凋亡、线粒体功能及结构解析中的应用。
**Background of PLSCR3 Recombinant Protein**
Phospholipid scramblase 3 (PLSCR3) is a member of the phospholipid scramblase family, which plays a critical role in mediating the bidirectional movement of phospholipids across cell membranes. This process is essential for maintaining membrane asymmetry, a feature crucial for cellular functions such as apoptosis, blood clotting, and immune signaling. Unlike other scramblases (e.g., PLSCR1), PLSCR3 is primarily localized to mitochondria, where it participates in mitochondrial membrane dynamics, lipid metabolism, and organelle homeostasis.
The recombinant PLSCR3 protein is engineered through molecular cloning and expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties and biological roles. Researchers utilize recombinant PLSCR3 to investigate its involvement in mitochondrial-associated processes, including membrane remodeling during apoptosis, mitophagy, and interactions with mitochondrial proteins like cardiolipin. Dysregulation of PLSCR3 has been linked to pathological conditions, such as cancer, where its overexpression may influence tumor cell survival and chemoresistance.
Structural studies of recombinant PLSCR3 reveal conserved domains, such as a DNA-binding N-terminal region and a C-terminal transmembrane segment, which facilitate its scramblase activity and membrane association. Its recombinant form enables *in vitro* assays to explore calcium-dependent phospholipid scrambling, protein-lipid interactions, and regulatory mechanisms.
PLSCR3's unique mitochondrial localization and functional versatility make it a compelling target for understanding metabolic disorders, neurodegenerative diseases, and cancer biology. Recombinant PLSCR3 serves as a vital tool for unraveling its physiological significance and potential therapeutic applications.
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